The mechanism of DHA's effect on the migration and invasion of human glioma T98G cells
Aim To investigate the mechanism of the effects of dihydroartemisinin(DHA)on migration and invasion of hu-man glioma T98G cells.Methods Human glioma T98G cells were cultured in vitro,and were divided into control group,low DHA group(10 mg/L DHA),medium DHA group(20 mg/L DHA),high DHA group(40 mg/L DHA),positive drug group(50 mg/L 5-fluorouracil)and inhibitor group(20 mg/L DHA+20 μmol/L LY294002).CCK-8,cell adhesion assay,Transwell chamber and Western blotting were used to analyze cell proliferation,adhesion,migration,invasion,EMT and phosphatidylinositol 3-kinase(PI3K)/seronine protein kinase(Akt)path-related protein expression levels in each group,respectively.Results With the cell processing time increased,the proliferation activity of T98G cells in each group showed a trend of increase(P<0.05).Compared with the control group,the proliferation activity,adhesion,migration and invasion ability,and the expression of p-PI3K,p-Akt,p-ERK,N-cadherin,Vimentin,and FN protein of T98G cells in other groups were significantly decreased(P<0.05),while the expres-sion of E-cadherin protein is significantly increased(P<0.05),and the low,medium,and high DHA groups showed a dose-dependent decrease or increase(P<0.05).Compared with the low and medium DHA groups,the proliferation activity,adhesion,migration and invasion ability,and the expression of p-PI3K,p-Akt,p-ERK,N-cadherin,Vimentin,and FN protein of T98G cells in the posi-tive drug group and inhibitor group were significantly decreased(P<0.05),while the expression of E-cadherin protein was significantly increased(P<0.05).Conclusion DHA inhibits adhesion,migration,invasion and EMT of human glioma T98G cells,possibly by inhibiting the signal transduction of PI3K/Akt pathway.
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