Ginsenoside Rg1 delayed hippocampal aging in mice after X-ray cranial irradiation by inhibi-ting TTR
Aim To investigate the potential mechanisms by which ginsenoside Rg1 delays X-ray cranial irradiation-induced hippocampal senescence in mice.Methods 40 C57BL/6J mice were randomly divided into the control group(Con group),the cranial irradiation group(IR group),the Rg1 pretreatment group(Rg1 group),and the Rg1 pretreatment plus cranial irradiation group(Rg1+IR group).The general health status of the mice,including body weight,food intake,activity,mental state,and fur and excreta,was observed and evaluated.Cognitive function was assessed by using the Morris water maze test,and the number of se-nescent cells in the hippocampal dentate gyrus was detected by β-galactosidase staining.RNA sequencing(RNA-seq)analysis was used to determine the target of Rg1 against radiation senescence.qRT-PCR and Western blotting were used to detect the expression levels of the targets.Results Compared with the Con group,the IR group showed decreased body weight,reduced food intake,decreased activity,mental fatigue,dull fur,and loose stools,as well as significant cognitive impairment and increased hippocampal dentate gyrus senescent cells(P＜0.05).Compared with the IR group,the Rg1+IR group exhibited improvements in all the afore-mentioned indicators(P＜0.05).Transthyretin(TTR)was identified as the most significant target.The expression of TTR in the hippocampus of IR group mice was increased compared with the control group(P＜0.05),while the Rg1+IR group was decreased com-pared with the IR group(P＜0.05).Conclusion Ginsenoside Rg1 attenuated X-ray-induced hippocampal senescence and protec-ted cognitive function in mice by suppressing the expression of TTR.
cranial irradiationginsenoside Rg1TTRhippocampalsenescencecognitive function
万方数据
维普
