首页|沙眼衣原体T3SS效应蛋白CT622通过激活ERK/p38 MAPK信号通路抑制Hela299细胞凋亡

沙眼衣原体T3SS效应蛋白CT622通过激活ERK/p38 MAPK信号通路抑制Hela299细胞凋亡

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目的 探究沙眼衣原体Ⅲ型分泌系统(T3SS)效应蛋白CT622对Hela299细胞凋亡的影响机制。方法 设置不同质量浓度CT622蛋白刺激Hela299细胞，筛选最佳刺激质量浓度。将细胞实验分为PBS组(空白对照)、肿瘤坏死因子(TNF)-α组(阳性对照)、CT622组、CT622+TNF-α组、CT622+PD98059(ERK1/2抑制剂)组、CT622+SB203580(p38 抑制剂)组、CT622+TNF-α+PD98059 组、CT622+TNF-α+SB203580 组。Hoechst33258 染色和流式细胞术检测Hela299细胞的凋亡情况。Western blotting检测凋亡相关蛋白裂解的Caspase-3(cleaved-Casepase-3)、B细胞淋巴瘤(Bcl)-2、Bcl-2相关X蛋白(Bax)和凋亡信号通路中信号分子ERK/p38 MAPK蛋白的表达。结果 5 mg/L CT622蛋白为Hela299细胞最佳刺激质量浓度。CT622蛋白显著抑制Hela299细胞的凋亡，并上调ERK1/2、p38 MAPK和Bcl-2蛋白表达，下调Bax和Caspase-3蛋白表达。分别利用ERK1/2和p38 MAPK抑制剂与CT622联合处理可以明显增加Hela299细胞凋亡程度。结论 T3SS效应蛋白CT622通过激活ERK/p38 MAPK信号通路抑制Hela299细胞凋亡。
Chlamydia trachomatis T3SS effector protein CT622 inhibits Hela299 cell apoptosis by acti-vating ERK/p38 MAPK signaling pathway
Aim To investigate the mechanism of Chlamydia trachomatis type 3 secretion system(T3SS)effector protein CT622 to affect Hela299 cell apoptosis.Methods Different mass concentrations of CT622 protein were used to stimulate Hela299 cells and to screen for the optimal stimulation mass concentration.Cell experiments were divided into PBS group(blank control),tumor necrosis factor(TNF)-α group(positive control),CT622 group,CT622+PD98059(ERK1/2 inhibitor)group,CT622+SB203580(ERK1/2 inhibitor)group,CT622+TNF-α+PD98059 group,and CT622+TNF-α+SB203580 group.The apoptosis of He-la299 cells was detected by Hoechst33258 assay and flow cytometry.The expression of apoptosis related proteins cleaved Caspase-3(cleaved-Caspase-3),B-cell lymphoma(Bcl-2),Bcl-2 associated X protein(Bax),and ERK/p38 MAPK protein were detected by Western blotting.Results The optimal mass concentration of CT622 protein to stimulate Hela299 cells were 5 mg/L.CT622 significantly inhibited the apoptosis of Hela299 cells,up-regulated the expression of ERK1/2,p38MAPK and Bcl-2,and down-regula-ted the expression of Bax and Caspase-3 proteins.The combination of ERK1/2 and p38 MAPK inhibitors with CT622 could signifi-cantly increase the apoptosis of Hela299 cells.Conclusion T3SS effector protein CT622 inhibits Hela299 cell apoptosis by acti-vating ERK/p38 MAPK signaling pathway.

Chlamydia trachomatisCT622 effector proteinMAPK signal pathwayapoptosis

胡艺宝、彭彬峰、曹倩、雷文波、刘双全

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南华大学衡阳医学院附属第一医院检验医学中心 微生物与感染性疾病研究所,湖南衡阳 421001

湖南中医药大学第一附属医院医学检验与病理中心,湖南长沙 410000

沙眼衣原体 CT622效应蛋白 ERK/p38 MAPK信号通路 细胞凋亡

湖南省自然科学基金湖南省自然科学基金湖南省高发性传播疾病精准诊治临床医学研究中心项目湖南省研究生科研创新项目南华大学临床医学研究"4310"项目

2022JJ305322023JJ305402023SK4051CX2023099920224310NHYCG02

2024

10.15972/j.cnki.43-1509/r.2024.03.003

中南医学科学杂志
南华大学

中南医学科学杂志

CSTPCD
影响因子：0.757
ISSN：2095-1116
年,卷(期)：2024.52(3)