Effects of miR-506 on macrophage migration inhibitory factor and intestinal barrier function in rats with acute pancreatitis
Aim To investigate the effects of microRNA-506(miR-506)on macrophage migration inhibitory factor(MIF)and intestinal barrier function in acute pancreatitis(AP)rats.Methods Fifty SPF grade SD rats were selected to establish AP model and treated with miR-506 and MIF.Pancreatic histopathological changes,intestinal barrier function[serum amylase,endotoxin,plasma D-lactic acid,lactulose/mannitol exclusion rate(L/M),bacterial translocation rate]and MIF level were observed.Results HE staining showed that the control group had normal pancreatic tissue morphology and structure,with no obvious pathological changes.Model group,MIF group,miR-506 group and miR-506+MIF group all had different degrees of pancreatic necrosis,and the lesions in MIF group were the most serious.miR-506 group had the mildest pathological changes.The pancreatic pathological score,serum am-ylase,endotoxin,MIF,plasma D-mammary gland level,lactulose/mannitol exclusion rate(L/M),bacterial translocation rate and MIF mRNA relative expression level of miR-506 group were lower than those of other groups,while the relative expression level of miR-506 was increased(P＜0.05).After dual intervention with miR-506 and MIF,serum amylase,endotoxin,MIF,plasma D-mammary gland lev-el,L/M,bacterial migration rate and relative expression level of MIF mRNA in miR-506+MIF group were higher than those in miR-506 group,and the relative expression level of miR-506 was lower than that in miR-506 group(P＜0.05).Conclusion The high expression of miR-506 alleviates the lesions of AP rats and improves the intestinal barrier function,which may be related to the inhibition of MIF.
acute pancreatitismiR-506macrophage migration inhibitor factorintestinal barrier function
万方数据
维普
