Ligusticum chuanxiong polysaccharide alleviates myocardial ischemia reperfusion injury in rats via AMPK/Nrf2 signaling pathway
Aim To investigate the effects of ligusticum chuanxiong polysaccharide(LCPS)on myocardial ischemia reperfu-sion injury(MIRI)and the roles of adenosine monophosphate-activated protein kinase(AMPK)/nuclear factor-erythroid 2-related fac-tor 2(Nrf2)signaling pathway in rats.Methods 120 SD rats were randomly divided into sham operation group,model group,LCPS group,AMPK activator AICAR group and LCPS+AICAR group.Except for the sham surgery group,other groups were used to construct MIRI rat models.The cardiac function indexes were detected by echocardiography,the content of creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH),myocardial troponin(cTnI)in serum were determined by ELISA,the cardiac index was alculated;the myocardial infarction size was detected by TTC staining,the myocardial histopathological changes were observed by HE staining,the content of malondialdehyde(MDA)and the activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)were determined by spectrophotometry,and the protein expression of AMPK,phosphorylation(p)-AMPK,Nrf2,heme oxygenase-1(HO-1)were detected by Western blotting.Results LCPS can significantly improve cardiac function and myocardial tissue dam-age in MIRI rats,reduce myocardial infarction area,MDA levels,cardiac index,serum LDH,CK-MB,cTnI levels,increase SOD,GSH-Px activity,p-AMPK/AMPK ratio,and Nrf2 and HO-1 expression levels.The impact of LCPS combined with AICAR on vari-ous indicators is larger than that of the LCPS group and AICAR group(P＜0.05).Conclusion LCPS can reduce the MIRI in rats,and the mechanism may be related to the activation of AMPK/Nrf2 signaling pathway and the inhibition of oxidative stress.
万方数据
维普
