Effects of MSC-AS1 on the biological behavior of cervical cancer cells and the mechanism
Aim To explore the role and mechanism of long non-coding RNA(lncRNA)MSC-AS1 in the biological behavior of cervical cancer cells.Methods qRT-PCR and Western blotting were used to detect the expression of MSC-AS1,miR-520d-3p and ATAD2 in cervical cancer tissues and SiHa cells.SiHa cells were divided into si-NC group,si-MSC-AS1 group,vector group,pc3.1-MSC-AS1 group,miR-NC group,miR-520d-3p group,si-NC+anti-miR-NC group,si-MSC-AS1+anti-miR-NC group,and si-MSC-AS1+anti-miR-520d-3p group.Flow cytometry,and Transwell experiment were used to evaluate the changes of cell apop-tosis,and migration and invasion levels,respectively.The targeted regulatory relationships among MSC-ASI,miR-520d-3p,ATAD2 were analyzed by combining starbase software and targetscan software with luciferase activity experiments.Results Com-pared with adjacent tissues,the expression of MSC-AS1 and ATAD2 protein in cervical cancer tissues were upregulated,while miR-520d-3p was downregulated(P＜0.05).After silencing MSC-AS1,the expression of miR-520d-3p and apoptosis rate of SiHa cells were higher in the si-MSC-AS1 group compared with the si-NC group,and lower in the pc3.1-MSC-AS1 group compared with the vector group after overexpression of MSC-AS1.After silencing MSC-AS1 while inhibiting miR-520d-3p,the expression of miR-520d-3p and apoptosis rate of SiHa cells were in an order of si-NC+anti-miR-NC group＜si-MSC-AS1+anti-miR-520d-3p group＜si-MSC-AS1+anti-miR-NC group(P＜0.05).MSC-AS1 targets miR-520d-3p,while miR-520d-3p targets ATAD2,and the apoptosis rate of SiHa cell in miR-520d-3p group were higher than in the miR-NC group(P＜0.05).The ATAD2 protein levels,migration number,invasion number of SiHa cells were lower in the si-MSC-AS1 group than in the si-NC group,higher in the pc3.1-MSC-AS1group than in the vec-tor group,ad lower in the miR-520d-3p group than in the miR-NC group,in an order of si-NC+anti-miR-NC group＞si-MSC-AS1+anti-miR-520d-3p group＞si-MSC-AS1+anti-miR-NC group(P＜0.05).Conclusion Silencing MSC-AS1 may target ATAD2 through miR-520d-3p to inhibit the growth and metastasis of cervical cancer cells,and promote apoptosis.
万方数据
维普
