Relationship between DNA methylation level of interferon-γ gene and no/low immunity to hepatitis B vaccine in children
Aim To investigate the relationship between DNA methylation level of interferon γ(IFN-γ)gene and no/low immunity of hepatitis B vaccine in children.Methods A total of 214 children aged 8-9 months with hepatitis B vaccination completed in 0,1 and 6 months were selected.According to the level of HBsAb,they were divided into no/low response group and normal/high response group.The methylation levels of 10 CpG site of the IFN-γ gene in the two groups were compared,and its as-sociation with no/low immune response to hepatitis B vaccine was analyzed.Results Of the 214 children,6 were non-respond-ers,81 were low responders,52 were normal responders,and 75 were high responders.The methylation levels of IFN-γ gene at sites of 44 and 93 in the non-/low-response group were higher than those in the normal/high-response group(P＜0.05).Logistic regression analysis showed that the high hypermethylation levels of IFN-γ gene at loci of 44[OR=1.320(1.031,1.934)]and 93[OR=1.480(1.146,2.374)]were child-related risk factors for no/low response to hepatitis B vaccine(P＜0.05).Conclu-sion The DNA methylation levels of IFN-γ gene at site of 44 and 93 are risk factors for no/low immune response to hepatitis B vac-cine in children.
万方数据
维普
