Riluzole improves motor function and muscle fibrosis in amyotrophic lateral sclerosis mice
Aim To explore the effects of Riluzole on motor function and muscle fibrosis in mice with amyotrophic lateral sclerosis(ALS).Methods 13 weeks old male transgenic ALS mice were selected and randomly divided into model group and Ri-luzole group.In addition,6 wild-type male mice were selected as the normal group.Motor ability of mice in each group were evalu-ated through suspension tests,pole climbing tests,pole rotation tests,grip strength tests and gait test.The body weight and serum creatine kinase and lactate dehydrogenase levels in each group of mice were compared.muscle histopathology was evaluated by muscle atrophy.Spinal cord lesions in each group were analyzed by immunohistochemistry.Results Compared with the normal group,the weight of ALS mice in model group were decreased significantly,and their motor function was significantly weakened,show-ing as increased climbing time,decreased hanging time and forelimb grasping power,shortened stride length,and elevated serum bio-chemical indexes creatine kinase and lactate dehydrogenase levels.Cellular infiltration and tissue fibrosis in skeletal myositis were ob-served,motor neurons in spinal cord were losted,and astrocytes and microglia were activated abnormally.Compared with the model group,the motor function of ALS mice in Riluzole group was significantly improved,the infiltration and fibrosis of inflammatory cells in skeletal muscle were reduced,the number of motor neurons lost in spinal cord was reduced,and the abnormal activation of astrocytes and microglia was inhibited.Conclusion Early treatment with Riluzole can improve motor dysfunction,gait retardation and mus-cle fibrosis in ALS mice.
万方数据
维普
