Protection of irisin on sarcopenia mice by up-regulating Wnt/β-catenin pathway
Aim To explore the mechanism of irisin in treating sarcopenia mice.Methods Male SPF wild C57 mice were randomly divided into the control group,model group,irisin group,irisin+Wnt/β-catenin pathway inhibitor group(irisin+XAV939 group),with 28 mice in each group.The level of behavioral indexes of mice in each group was detected by grasping force detection,rope grasping time and swimming experiment.The wet to weight ratio of gastrocnemius muscle in each group was com-pared.HE staining was used to observe the pathological changes of gastrocnemius muscle in each group.The expression levels of autophagy-related proteins(Beclin1,LC3 Ⅱ/Ⅰ)and pathway-related proteins(Wnt,β-catenin)were detected by Western blotting.Real-time fluorescence quantitative PCR was used to detect the expression levels of mitotic fusion related-genes,such as dynamic relat-ed protein 1(Drp1),mitochondrial fusion protein 2(Mfn2)and optic atrophy protein 1(Opa1).Results In the control group,the gastrocnemius tissue was arranged neatly,and there was no inflammatory infiltration,edema or necrosis.In the model group,the gastrocnemius muscle tissue structure was seriously damaged,the tissue arrangement was disordered,and a large number of inflamma-tory cells infiltrated and muscle fibers dissolved.The gastrocnemius tissue in irisin group was significantly improved compared with those in model group.Irisin+XAV939 group is similar to the model group.Compared with the control group,the grasping power,the expression of Beclin1,LC3 Ⅱ/Ⅰ,Drp1 mRNA,Wnt and β-catenin in the model group were decreased(P＜0.05),the time of grasping rope and swimming,the wet to weight ratio of gastrocnemius were decreased(P＜0.05),and the expressions of Mfn2 and Opa1 mRNA were increased(P＜0.05).Compared with the model group,the levels of the above indicators in the irisin group were reversed(P＜0.05).Compared with the irisin group,the changes in the above indicators in the irisin+XAV939 group showed the same trend as the model group(P＜0.05).Conclusion Irisin may improve autophagy,promote mitochondrion division and inhibit mitochondrion fusion in mice with sarcopenia by up-regulating Wnt/β-catenin pathway,thus playing a certain role in muscle protection.
万方数据
维普
