Mechanism of the effect of SVT on inflammatory response and ectopic endometrial cell apop-tosis in EM rats
Aim To Explore the effect of simvastatin(SVT)on inflammatory response and apoptosis of ectopic endometrial cells in rats with endometriosis(EM).Methods 48 SD female rats were randomly divided into the control group,the EM group,the SVT group and the SVT+lipopolysaccharide(LPS)group.Histological analysis was used to detect the volume and adhesion score of endometriosis lesions in each group of rats.ELISA kits were used for detecting interleukin-6(IL-6),vascular endothelial growth factor(VEGF),and tumor necrosis factor-α(TNF-α)in rat serum.HE staining was used to observe the pathological changes of rat endometrium tissue.TUNEL staining was used to detect endometrial cell apoptosis.Western blotting method was used to detect TNF-α/nuclear factor-κB(NF-κB)pathway related proteins.Results Compared with the control group,rats in EM group showed distorted intimal structure and obvious inflammatory cell infiltration,along with increased endometriotic lesion volume and ab-dominal adhesion score,increased serum IL-6,VEGF and TNF-α levels,enhanced ectopic endometrial cell apoptosis and TNF-α/NF-KB signal pathway activation(P＜0.05).Compared with the EM group,the SVT treatment significantly restored the endometrial epi-thelial structure,and decreased the volume of endometriosis lesions and abdominal adhesion score,as well as serum IL-6,VEGF and TNF-α levels were decreased.TNF-α/NF-κB signal pathway was inhibited,whereas ectopic endometrial cell apoptosis was further enhanced(P＜0.05).The apoptosis index of SVT+LPS group was decreased compared with the SVT group(P＜0.05).Conclu-sion Simvastatin reduces the inflammatory response in EM rats and promotes apoptosis of ectopic endometrial cells by inhibiting TNF-α/NF-κB signaling pathway.
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