circ_0068655 can promote apoptosis of cardiomyocyte HCM by sponging miR-498
Aim To investigate the effects of circular_0068655 on the apoptosis of cardiomyocytes.Methods Twelve male rats were randomly divided into Sham group and acute myocardial infarction group(MI group).qRT-PCR was used to detect the expression of circ_0068655 and miR-498,and Western blotting was performed to detect the expression of apoptosis-related proteins in myocardium of two groups of rats.HCM were divided into blank control group,circ_0068655 knockdown group(sh-circ_0068655 group),circ_0068655 overexpression group,hypoxia group(hypoxia group),knockdown control+hypoxia group(sh-ctrl+hypoxia group),circ_0068655 knockdown+hypoxia(sh-circ_0068655+hypoxia group).Hypoxic HCM were divided into knockdown control+miR-498-inhibitor-NC group(sh-ctrl+miR-498-inhibitor-NC group),circ_0068655 knockdown+miR-498-inhibitor-NC group(sh-circ_0068655+miR-498-inhibitor-NCgroup)and circ_0068655 knockdown+miR-498-inhibitor group(sh-circ_0068655+miR-498-inhibitor group).The expression levels of circ_0068655 and miR-498 in HCM were detected by qRT-PCR,and cell activity,apopto-sis,migration and invasion were detected by CCK-8,ELISA and Transwell methods in all groups.The binding relationship between circ_0068655 and miR-498 was analyzed by RNA pull-down and dual luciferase assays.Results The expression of circ_0068655 in myocardial tissue of MI rats was higher than that of Sham group,and the expression of miR-498 was lower than that of Sham group.Knockdown of circ_0068655 could improve the viability of HCM cardiomyocytes induced by hypoxia,improve the migration and inva-sion ability of cardiomyocytes,and reduce the apoptosis rate and apoptosis-related protein expression.circ_0068655 can bind and negatively regulate the expression of miR-498.miR-498-inhibitors can inhibit the protective effects of circ_0068655 knockdown on hypoxic cardiomyocytes.Conclusion circ_0068655 can promote the apoptosis of cardiomyocytes in myocardial infarction by spon-ging miR-498.
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