Curcumin regulates synaptic plasticity upregulating GRIA1 to counteract cognitive impairment in Parkinson's disease
Aim To explore the effect of curcumin on cognitive impairment in Parkinson's disease(PD)by upregulating GRIA1 to regulate synaptic plasticity.Methods 4 rats were selected as control group.20 rats were established a rat model of Parkinson's disease(PD)with cognitive impairment by unilaterally injecting 6-OHDA into the striatum,and the mice were randomly divided into PD group,PD+Cur group,PD+LY341495 group and PD+Cur+LY341495 group.The learning and memory abilities of the rats were assessed by using the Y-maze and Morris water maze tests.Immunofluorescence staining was performed to observe the quantity of postsynaptic density protein 95(PSD95)and AMPA receptors(GluR2)in the striatum.Western blotting and qRT-PCR were conducted to measure the expression of glutamate ionotropic receptor AMPA type subunit 1(GRIA1)protein and mRNA.Results Compared with the control group,the PD group showed a decrease in correct replacement rate,expression of PSD95 and GluR2,prolonged escape latency,decreased number of passes through the platform,target quadrant residence time,GRIA1 protein and mRNA expression,and an increase in the number of Lewy bodies(P＜0.05).Compared with the PD group,the PD+Cur group showed an increase in correct replacement rate,expression of PSD95 and GluR2,a decrease in escape latency,an increase in platform frequency,target quadrant residence time,GRIA1 protein and mRNA expression,and a decrease in the number of Lewy bodies.The trend of changes in the PD+LY341495 group and the PD+Cur+LY341495 group was opposite to that in the PD+Cur group(P＜0.05).Conclusion Curcumin can counteract cognitive impairment in Parkinson's disease(PD),and its mechanism may involve the modula-tion of synaptic plasticity through the upregulation of GRIA1.
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