miR-21-5p ceRNA lncRNA MEG3 inhibits cell proliferation and invasion in TE11 esophageal squamous cell carcinoma
Aim To detect the expression level of lncRNA MEG3 and miR-21-5p in ESCC tissues and cells,and further ex-plore whether MEG3 could function as a competing endogenous RNA(ceRNA)of miR-21-5p to inhibit ESCC cells proliferation and in-vasion.Methods Paired fresh cancer tissues and adjacent tissues were taken from 42 surgical patients with esophageal cancer.The expression of lncRNA MEG3 and miR-21-5p in tissues was examined by quantitative real-time PCR(qRT-PCR).The expression of lncRNA MEG3 and miR-21-5p in each cell line was detected by qRT-PCR.A stable cell line overexpressing MEG3 gene was con-structed.CCK-8 and transwell assays were applied respectively to detect the ESCC cell proliferation and invasion.The targeting re-lationship between lncRNA MEG3 and miR-21-5p was studied by luciferase assay.Anti-AgO2 RNA binding protein immunoprecipita-tion(RIP)experiment was used to verify whether MEG3 and miR-21-5p can be enriched in the immunoprecipitate.Results ln-cRNA MEG3 was decreased in ESCC tissues compared with paracancerous tissues(P＜0.05).On the contrary,miR-21-5p was over-expressed in ESCC tissues compared with paracancerous tissues(P＜0.05).Their expression in ESCC was negatively correlated(P＜0.05).The low expression of MEG3 was positively correlated with large tumor size,worse differentiation,advanced stage and lymph node metastasis.Overexpression of MEG3 can negatively regulate the expression of miR-21-5p(P＜0.05),and can inhibit the prolif-eration and invasion of TE11 cells(P＜0.05).Overexpression of miR-21-5p in MEG3 overexpression TE11 cells weakened the inhi-bition of cell proliferation and invasion through the overexpression of MEG3(P＜0.05).Luciferase assay showed that infection of miR-21-5p could significantly reduce the luciferase activity of cells containing wild-type MEG3(P＜0.05).RIP experiments showed that lncRNA MEG3 and miR-21-5p were significantly enriched in the immunoprecipitate of AgO2(P＜0.05).Conclusion lncRNA MEG3 is an ESCC tumor suppressor gene.As a miR-21-5p ceRNA,lncRNA MEG3 inhibits the proliferation and invasion of TE11 ESCC cells.
万方数据
维普
