Effects of microcystin-LR on liver glucose and lipid metabolism in mice
Aim To investigate the effects of microcystin-LR(MC-LR)on liver glucose and lipid metabolism in mice.Methods A total of 40 male C57BL/6 mice were randomly divided into control group,low toxicity group,medium toxicity group and high toxicity group,and were given drinking water containing 0,1,60 and 120 μg/L MC-LR for 6 months,respectively.Except the control group,the in vivo model of chronic MC-LR infection were constructed in other groups.The histopathological changes of liver were observed by HE and oil red O staining.Levels of serum asprosin and insulin(INS)were detected by ELISA kits.The liver weight,liver index and serum levels of glutamic-pyruvic transaminase(GPT),glutamic-oxaloacetic transaminase(GOT),blood sugar,triglyceride(TG),total cholesterol(TC),free fatty acid(FFA),high density lipoprotein cholesterol(HDLC)and low density lipoprotein cholesterol were compared among all groups.Results There was no significant difference in liver body mass and liver coefficient between the poisoned groups of mice and the control group(P＞0.05).The results of HE and oil red O staining showed that the liver lobule structure of mice of control group and low toxicity group was contact,liver cells were arranged in normal or-der,and no obvious pathological changes were observed.Hepatic lobule arrangement was disordered in the medium and high toxicity groups,with a small amount of inflammatory cell infiltration and steatosis observed,and the degree of steatosis was the most serious in the high toxicity group.Compared with the control group,the levels of GPT,GOT,TG,TC,FFA,asprosin,INS and blood sugar in medium and high toxicity groups were increased(P＜0.05),while the levels of HDLC were decreased(P＜0.05).Conclusion The exposure of MC-LR can induce disorder of liver glucose and lipid metabolism in mice.
万方数据
维普
