The effects of hypoxic-ischemic brain injury on the expression of AQP-9 and spatial learning and memory ability in neonatal mouse brain tissue
Aim To explore the effects of hypoxic ischemic brain injury on the expression of AQP-9 and spatial learning and memory abilities in the brain tissue of neonatal mice.Methods Neonatal mice with hypoxic-ischemic encephalopathy(HIE)born from pregnant mice with blocked uterine arteries were included in the HIE group;Mice born from pregnant female mice without bloc-king uterine arteries were included in the control group.The distribution of neurons between two groups was compared.Immunohis-tochemical method was used to detect the expression of small albumin in the CA1,CA3,and DG regions of mouse hippocampus.Western blotting was used to detect the expression of neurotransmission-related proteins NR2B,EAAT1,AQP-9,and glutamic acid de-carboxylase(GAD)in the hippocampus of mice.New object recognition experiments were used to test the spatial learning and memo-ry abilities of mice.Results Compared with the control group,the neuron density in the CA1 and CA3 regions of the HIE group were decreased(P＜0.05),but this change showed no significant difference compared with the DG region(P＞0.05).The expres-sion of small albumin in all three regions of the HIE group was higher than that of the control group(P＜0.05).The expression levels of AQP-9 and GAD in the HIE group were higher than those in the control group(P＜0.05).The HIE group mice have spatial learn-ing and memory deficits.Conclusion The expression level of AQP-9 in the brain tissue of HIE newborn mice increases,and their spatial learning and memory abilities decrease.
hypoxic ischemic encephalopathyspatial learning and memory abilityAQP-9mouse
万方数据
维普
