Inhibition and clearance effects of bacteriophage LLY combined with levofloxacin on the bio-film of extensively drug-resistant Acinetobacter baumannii ABLL
Aim To explore the effects of bacteriophage LLY combined with levofloxacin(LEV)on the control of extensively drug-resistant Acinetobacter baumannii ABLL biofilm.Methods The biofilm experiment was divided into different concentrations of LLY groups,different mass concentrations of LEV groups,different concentrations of LLY combined with different mass concentrations of LEV groups,and control group.Crystal violet staining method was used to detect the biomass of biofilm.MTT method was used to detect the viability of biofilm cells.The structure of biofilm was observed by microscopic imaging method.The diameter of plaque under LEV was measured by the double layer agar plate method.ABLL-induced prophage release carried by LEV was detected by spot lysis test.Results Compared with the control group,the combination of 106 PFU/mL LLY+8 mg/L LEV group showed a decrease in the ABLL biofilm biomass and the viability of biofilm bacteria(P＜0.05),and the reduction effect was better than that of the 8 mg/L LEV group and the 106 PFU/mL LLY group(P＜0.05).Furthermore,the combination group showed significant disruption of the reticular structure of the biofilm.The diameter of the phage plaques was increased in the LEV group compared with the group without LEV(P＜0.05).LEV could induce the release of prophages from ABLL.Conclusion The combination of bacteriophage LLY and LEV could effectively prevent or eradicate the biofilm of extensively drug-resistant Acineto-bacter baumannii ABLL.
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