Effects of leaving hypobaric and hypoxic environment on pulmonary vascular reconstruction and TNF-α expression in rats with high altitude pulmonary hypertension
Aim To explore the effects of leaving hypobaric and hypoxic environment on pulmonary vascular reconstruction and tumor necrosis factor α(TNF-α)expression in rats with high altitude pulmonary hypertension.Methods A total of 120 Wistar rats were randomly divided into control group,high-altitude group(10 d,20 d,30 d)and low-altitude group(10 d,20 d,30 d,90 d).The pulmonary artery pressure and right ventricular hypertrophy indexes were detected.The pathological changes of lung tissues were observed by HE staining,the degree of pulmonary vascular reconstruction[the percentage of pulmonary arteriole wall thickness to outer diameter(WT％),and the percentage of tube wall area to total area(WA％)]was evaluated,and expression of TNF-α in lung tissue sections was detected by immunohistochemistry.Results Compared with control group,mean pulmonary ar-tery pressure(mPAP),right ventricular hypertrophy index(RVHI),WT％,WA％and expression of TNF-α were increased in high-altitude group.Compared with high-altitude 30d group,mPAP,RVHI,WT％,WA％and expression of TNF-α were decreased in low-altitude group.With time extension,the above changes were more significant(P＜0.05).With the extension of molding time,thickness of pulmonary vascular wall was gradually thickened,and lumen was gradually decreased.By using 30d molding in high alti-tude area as reference to low altitude group,with the extension of returning low altitude area time,thickness of pulmonary vascular wall was gradually thinned,lumen was gradually increased(P＜0.05).Conclusion After leaving hypobaric and hypoxic environment,right ventricular hypertrophy and pulmonary vascular reconstruction are improved,and expression of TNF-α in lung tissues is decreased in rats with high altitude pulmonary hypertension.
rat with high altitude pulmonary hypertensionhypobaric and hypoxicpulmonary vascular reconstructiontumor necrosis factor-α
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