In order to evaluate the effects of culture medium compositions(carbon source, nitrogen source, growth factors etc.) on the growth rate of yeast, the main influencing factors were determined by Plackett-Burman design. On the basis of the most steep climb path experiment, the peak values of those factors were determined and those factors were then optimized by response surface method as follows:sucrose 58 g/L, apple juice 22 mL/L, peptone 12 g/L, potato juice 10 mL/L, yeast powder 10 g/L, calcium chloride 0.1 g/L, dipotassium hydrogen phosphate 1 g/L, and the growth rate of yeast was 8.8748 cfu/mL after 24 h culture at 28℃and pH 4.5. Compared with traditional YPD culture medium (8.5543 cfu/mL), yeast quantity in the optimized culture medium increased by 2 times.
NETL
NSTL
万方数据
维普
