Present Status in Research Technology of Liquor-making Microbial Communities & Next-generation Sequencing Data Analysis
Liquor-making is essentially the process of microbial communities succession and microbial metabolites accumulation and transfor-mation. To reveal the mystery of liquor, we should start from microbial research. Microbes in communities are, in general, advancing the fer-mentation of grains. The analysis of the composition and the succession of microbes is not only the premise to evaluate the yield and the quality of liquor, but also the basis for exploring the functions and metabolites of microbes. In this paper, aiming at the current application status of mi-crobial research technology in liquor-making field, we found that culture-based approach and culture-independent DGGE/TGGE techniques are mainly applied in microbial community research nowadays, while next-generation sequencing technique is rarely adopted (reported only in for-eign journals). Researchers not capable of understanding or manipulating the sequencing data might be the main reason for the poor use of next-generation sequencing technique. In view of such problem, systematical and straightforward interpretation of sequencing data were made in this review. Furthermore, five categories of multivariate analysis which could deeply and comprehensively present the microbial information were recommended, and we also provided the strategies and usages of these analytic approaches for reference.
NETL
NSTL
万方数据
维普
