酱香型白酒轮次基酒中乳酸检测方法的研究
Detection Method of Lactic Acid in Jiangxiang Crude Liquor of Different Production Rounds
尹雅洁 1冯洁 1肖瑶 1张星星 1张娅 1毕研芳 1李细芬 1卢君 1李长文1
作者信息
- 1. 贵州国台酒业集团股份有限公司,贵州仁怀 564501;贵州国台酒业集团研究院,天津 300410
- 折叠
摘要
通过轮次基酒乳酸检测对比实验,建立了高效液相色谱检测酱香型白酒轮次基酒乳酸的方法,为轮次基酒风味成分的检测提供了参考.采用PR-C18色谱柱(4.6×250 mm,5 μm),以0.1%磷酸水-甲醇为流动相,采取等度洗脱的方式,检测波长214 nm,流速1 mL/min,柱温40℃,进样量5 μL.方法学验证表明,该方法在特定线性范围内,乳酸有较好的线性关系,相关系数R2为0.9997,加标回收率为100%～110%,重复性、稳定性RSD均小于2%,可用于酱香型白酒不同轮次基酒的乳酸检测.
Abstract
Through comparative experiments,a method for the detection of lactic acid in Jiangxiang crude liquor of different produc-tion rounds was established by using high performance liquid chromatography(HPLC).The separation was performed on a PR-C18 column(4.6×250 mm,5 μm),the isometric elution was performed with 0.1%H3PO4-methanol solution as the mobile phase,the detec-tion wavelength was 214 nm,the flow rate was 1 mL/min,the column temperature was 40℃,and the injection volume was 5 μL.The methodological verification showed that lactic acid had good linear relationship in a specific linear range,the correlation coeffi-cient R2 was 0.9997,the recovery rate was between 100%and 110%,and the repeatability and stability RSD were less than 2%.The method can be used to detect lactic acid in Jiangxiang crude liquor of different production rounds.
关键词
轮次基酒/高效液相色谱/乳酸/酱香型白酒Key words
crude liquor of different production rounds/high performance liquid chromatography/lactic acid/Jiangxiang Baijiu引用本文复制引用
出版年
2024
NETL
NSTL
万方数据