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基于微生物固定化的浓香型白酒无窖泥发酵影响因素研究

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本实验以丝瓜络为载体,通过固定化窖泥中的功能微生物,从而代替窖泥发酵浓香型白酒.考察了载体添加量、加曲量、发酵时间、载体添加方式等因素对浓香型白酒无窖泥发酵酒醅及酒体中风味物质的影响,并利用冗余分析(redundancy analysis,RDA)方法确定了影响白酒中四大酯含量的主要因素.结果表明,不同因素条件对酒醅的含水率无明显影响,而酸度随着载体添加量、加曲量、发酵时间的增加呈现增加的趋势.在酒体中共检测到79种化合物,其中未添加固定化载体的酒体中检测到26种物质.结合冗余分析发现酒体中己酸乙酯含量与载体添加方式相关性最强,其次是发酵时间.丁酸乙酯与发酵时间呈正相关,而乙酸乙酯与载体添加量和加曲量呈正相关.因此,在后续的生产操作中可以适当调控发酵条件来改善酒体中四大酯的含量.本研究证明了丝瓜络固定复合己酸菌代替窖泥生产浓香型白酒具有实际可行性,为无窖泥发酵浓香型白酒的工艺生产提供了参考.
Study on Influencing Factors of Nongxiangxing Baijiu Fermentation Without Cellar-mud Based on Microbial Immobilization
In this experiment,Nongxiangxing baijiu was fermented by immobilizing functional microorganisms in cellar mud using loofah as carrier.The effects of carrier addition amount,yeast addition amount,fermentation time and carrier addition method on flavor substances in the fermented grains and body of Nongxiangxing baijiu without cellar mud were investigated.redundancy analysis(RDA)was used to determine the main factors affecting the contents of four esters in liquor.The results showed that the moisture content of fermented grains was not affected by different factors,but the acidity of fermented grains increased with the increase of the carrier addition amount,the fermentation amount and the fermentation time.A total of 79 compounds were detected in the wine body,of which 26 compounds were detected in the wine body without immobilized carrier.Combined with redundancy analysis,it was found that the ethyl caproate content in wine body had the strongest correlation with carrier addition method,followed by fermentation time.The ethyl butyrate was positively correlated with the fermentation time,while the ethyl acetate was positively correlated with the amount of carrier and curd.Therefore,in the subsequent production operation,the fermentation conditions can be properly regulated to improve the content of four esters in the wine body.This study proved that it is feasible to produce Nongxiangxing baijiu with complex caproic acid bacteria fixed by loofah Nongxiangxing baijiu instead of cellar mud,and provided a reference for the fermentation process of Nongxiangxing baijiu without cellar mud.

Nongxiangxing baijiuFermentation without mudImmobilizationFour estersRedundancy analysis

汪江波、荣光玉、杜彪、朱嘉璐、何超、张瑞景、蔡凤娇、徐健

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工业发酵省部共建协同创新中心,发酵工程教育部重点实验室,工业微生物湖北省重点实验室,湖北工业大学生命科学与健康工程学院,湖北 武汉 430000

湖北毕圣泉酒业有限公司,湖北 黄冈 438700

浓香型白酒 无窖泥发酵 固定化 四大酯 冗余分析

2025

酿酒
中国标准化协会 黑龙江轻工业科学研究院 黑龙江省酒业协会

酿酒

影响因子:0.422
ISSN:1002-8110
年,卷(期):2025.52(1)