Isolation,Identification and Application of Pyrazine Producing-Microorganism in Luzhou flavor Daqu
The high-temperature Daqu in Songhe was targeted in the program to isolate and optimize high-yield pyrazine producing microbial strain.Based on the morphological and physiological characteristics,combined with 16S rDNA analysis technology,the strain was identified as Bacillus licheniformis.The fermentation broth was analyzed by gas chromatography-mass spectrometry(GC-MS),and it was determined that the strain had a strong ability to produce tetramethylpyrazine.The enlarged cultivation of the strain was performed to produce reinforced Daqu,which was used in liquor production to increase the health and active factors of Baijiu,and to improve the style of liquor body and quality of Baijiu.