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浓香大曲中产吡嗪类微生物的分离鉴定及应用研究

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本项目主要以宋河中高温大曲为研究对象,从中分离优化出高产吡嗪类微生物菌株,根据菌株形态特征、生理生化特性,结合16S rDNA分析技术对菌株进行鉴定,确定该菌株为地衣芽孢杆菌.利用气相色谱-质谱联用技术(GC-MS)对发酵液进行分析,确定该菌株具有较强的产四甲基吡嗪的能力.对该菌株进行扩大培养制成强化大曲,用于酿酒生产,以增加白酒的健康活性因子,完善酒体风格,提高白酒质量.
Isolation,Identification and Application of Pyrazine Producing-Microorganism in Luzhou flavor Daqu
The high-temperature Daqu in Songhe was targeted in the program to isolate and optimize high-yield pyrazine producing microbial strain.Based on the morphological and physiological characteristics,combined with 16S rDNA analysis technology,the strain was identified as Bacillus licheniformis.The fermentation broth was analyzed by gas chromatography-mass spectrometry(GC-MS),and it was determined that the strain had a strong ability to produce tetramethylpyrazine.The enlarged cultivation of the strain was performed to produce reinforced Daqu,which was used in liquor production to increase the health and active factors of Baijiu,and to improve the style of liquor body and quality of Baijiu.

Luzhou flavor Daqustrain screeningstrain identificationtetramethylpyrazine

李学思、曹振华、李军、范馨予

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河南牧业经济学院食品与生物工程学院,河南 郑州 450046

河南省宋河酒业股份有限公司,河南 鹿邑 477200

浓香大曲 菌种筛选 菌种鉴定 四甲基吡嗪

2025

酿酒
中国标准化协会 黑龙江轻工业科学研究院 黑龙江省酒业协会

酿酒

影响因子:0.422
ISSN:1002-8110
年,卷(期):2025.52(1)