目的:探讨微小RNA(miRNA)-185表达与结直肠癌临床病理特点的关联及其对预后的评估价值.方法:回顾性分析手术治疗的130例结直肠癌患者的临床资料,使用荧光定量聚合酶链反应(PCR)技术检测癌组织标本和癌旁正常组织中的miRNA-185表达水平,分析不同临床病理资料特征下miRNA-185表达水平差异.通过受试者工作特征(ROC)曲线和Cox多因素回归分析miRNA-185对预后的评估价值.结果:结直肠癌组织中miRNA-185水平低于癌旁正常组织(0.176±0.085 vs 0.364±0.113,P＜0.05),不同性别、年龄、分化程度、肿瘤位置和肿瘤大小特征下miRNA-185表达水平无统计学差异(P均＞0.05),miRNA-185在Ⅲ～Ⅳ期结直肠癌、淋巴结转移肿瘤组织中表达水平更低(P均＜0.05).对评估术后肿瘤是否进展进行ROC曲线分析显示,miRNA-185的曲线下面积为0.816,95％CI:0.716～0.923,灵敏度为70.21％,特异性为83.23％,多因素Cox结果显示,TNM分期为Ⅲ～Ⅳ期(HR=2.417,95％CI:1.362～4.289),miRNA-185 低表达(HR=3.121,95％CI:1.603～6.077)是预后的危险因素.结论:miRNA-185在结直肠癌组织中表达水平下调,并能作为潜在的评估结直肠癌术后无进展生存的标志物.
Expression of miRNA-185:a potential marker of progression-free survival after colorectal cancer
Objective:To evaluate the expression of miRNA-185 in different clinicopathological characteristics of color-ectal cancer and its value as a prognostic marker.Methods:Totally,130 patients with colorectal cancer were retrospectively selected.The expression level of miRNA-185 was detected in cancer tissue specimens and normal tissues adjacent to cancer by fluorescence quantitative PCR.The miRNA-185 expression in different clinicopathological data was compared.Multivari-ate Cox regression and ROC curve were used to evaluate the prognostic value of miRNA-185.Results:The level of miRNA-185 in colorectal cancer tissues was significantly lower than that in normal tissues adjacent to cancer(0.176±0.085 vs.0.349±0.114,P＜0.05).The expression level of miRNA-185 was not significantly correlated with gender,age,degree of differentiation,tumor location and size(P＞0.05),but the expression was lower in stage Ⅲ-Ⅳ colorectal cancer and lymph node metastasis tumor tissues(P＜0.05).The ROC curve analysis of postoperative tumor progression showed that the area under the curve of miRNA185 was 0.816(95CI％:0.716-0.923),sensitivity was 70.21％,and specificity was 83.23％.Multivariate Cox analysis showed TNM stage Ⅲ-Ⅳ(HR=2.417,95％CI:1.362-4.289),miRNA-185 low expression(HR=3.121,95％CI:1.603-6.077)were prognostic risk factors.Conclusion:The expression of miRNA-185 is down-reg-ulated in colorectal cancer tissues and can be used as a potential marker of progression-free survival after colorectal cancer surgery.
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