Correlation between serum microRNA-124 and Caspase-9 and prognosis in patients with acute cerebral infarction
Objective:To explore the relationship between serum microRNA-124(miR-124)and cysteinyl aspartate spe-cific protease-9(caspase-9)in patients with acute cerebral infarction(ACI),and their predictive value for prognosis.Meth-ods:A total of 216 patients with ACI were collected.Meanwhile,86 healthy examinees were selected as the reference group.The level of serum miR-124 was detected by real-time fluorescence quantitative PCR(qRT-PCR),and the levels of Caspase-9,high-sensitivity C-reactive protein(hs-CRP),tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and Pentraxin(PTX)-3 were detected by enzyme linked immunosorbent assay(ELISA).According to the 6-month follow-up results,ACI patients were divided into a good prognosis group of 152 cases and a poor prognosis group of 64 cases.The correlation be-tween serum miR-124,Caspase-9 and inflammatory factors in poor prognosis group was analyzed.Receiver Operator charac-teristic(ROC)curve was applied to analyze the predictive value of serum miR-124 and Caspase-9 in the prognosis.Logistic regression was performed on prognostic factors.Results:The levels of miR-124,Caspase-9,hs-CRP,TNF-α,IL-6,PTX-3 in poor prognosis group were higher than those in good prognosis group and control group,the levels of low density lipoprotein cholesterol(LDL-C)and proportion of patients with coronary heart disease,diabetes,and large vessel occlusion in poor prognosis group were higher than those in good prognosis group(P＜0.05).The levels of serum miR-124 and Caspase-9 in patients with poor prognosis were positively correlated(r=0.582;P＜0.05),and they were positively correlated with hs-CRP,TNF-α,IL-6 and PTX-3(P＜0.05).The area under the curve(AUC)of serum miR-124 and Caspase-9 in predic-ting the prognosis of patients with ACI was 0.906 and 0.836,the specificity was 77.6％and 88.8％,and the sensitivity was 89.1％and 71.9％,respectively.The AUC of the two factors combination was 0.920,the specificity and the sensitivity was 90.8％,81.3％,respectively.MiR-124,Caspase-9,LDL-C,coronary heart disease,diabetes,and large vessel occlusion were the risk factors of poor prognosis in patients with ACI(P＜0.05).Conclusion:Serum miR-124 combined with Caspase-9 could contribute to early predicting the prognosis of patients with ACI.
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