Application of cutaneous tissue detected by flow cytometry in the diagnosis of blastic plasmacytoid dendritic cell neo-plasm
Objective:To investigate the value of flow cytometry(FCM)in the diagnosis of blastic plasmacytoid dendritic cell neoplasm(BPDCN)by detecting cutaneous tissue samples.Methods:The cutaneous tissue and bone marrow were detected by multi-color FCM,and the results of histopathology,bone marrow cell morphology,genetics,and second-generation sequencing were analyzed retrospectively in 3 cases of confirmed BPDCN.Results:All 3 patients had skin damage as the initial symptom.FCM detection of bone marrow only found BPDCN cells in one patient sample,while FMC detection of skin tissue samples found BPDCN cells in all 3 patients.The diagnosis was consistent with the pathological and immuno-histochemical diagnosis of skin tissue.The bone marrow cytology and genetic examination in one patient(case 2)did not detect BPDCN cells.BPDCN cells were found in bone marrow cytology of one patient(case 1),and BPDCN related gene mutations were detected in one patient(case 3)by second-generation sequencing.Conclusion:FCM immunophenotyping is a practical auxiliary tool for the diagnosis of BPDCN,which can quickly and accurately diagnose BPDCN in early skin tissue specimens,and perform differential diagnosis.It is an effective pathological auxiliary tool.
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