支链氨基酸有效预测慢性阻塞性肺病的急性加重
Branched-chain amino acids effectively predict acute exacerbation of chronic obstructive pulmonary disease
武小杰 1李伟 2毛莉娜 1杨硕1
作者信息
- 1. 武汉市第一医院呼吸与危重症医学科,湖北武汉 430022
- 2. 武汉市第一医院内分泌科,湖北武汉 430022
- 折叠
摘要
目的:探讨支链氨基酸(BCAAs)代谢在慢性阻塞性肺病(COPD)发生机制中的作用.方法:收集42例COPD稳定期患者纳入COPD组,40例COPD急性加重(AECOPD)期患者纳入AECOPD组,收集同期46例健康体检者为对照组,采集外周血,应用液相色谱-质谱分析检测BCAAs水平,酶联免疫吸附(ELISA)法检测炎症因子水平,并对二者的相关性进行分析.结果:与对照组比较,COPD组及AECOPD组患者血浆亮氨酸、缬氨酸水平更低,且AE-COPD组低于COPD组;血浆白介素(IL)-8、C-反应蛋白(CRP)水平更高,且AECOPD组高于COPD组(P均＜0.001).血浆中亮氨酸、缬氨酸水平与IL-8、CRP水平呈负相关(r=-0.64、-0.71、-0.73、-0.73,P均＜0.05).结论:BCAAs代谢可能通过炎症反应参与COPD的发生,临床中BCAAs水平有可能成为检测COPD急性加重潜在的生物标志物.
Abstract
Objective:To investigate the role of branched-chain amino acids(BCAAs)metabolism in the pathogenesis of chronic obstructive pulmonary disease(COPD).Methods:Totally,42 patients with stable COPD were included in the COPD group,40 patients with acute exacerbation of COPD served as the AECOPD group,and 46 healthy subjects were fallen into the control group.BCAAs levels in peripheral blood were detected by liquid chromatography tandem mass spectrometry and the levels of cytokines in peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA).The correla-tion between them was analyzed.Results:Compared with the control group,the serum leucine and valine levels in COPD group and AECOPD group were lower,and those in the AECOPD group were lower than those in the COPD group.Compared with the control group,the plasma levels of IL-8 and CRP in the COPD group and AECOPD group were increased,and those in the AECOPD group were higher than those in the COPD group(all P＜0.001).Plasma leucine and valine levels were nega-tively correlated with IL-8 and CRP levels(r=-0.64,-0.71,-0.73,-0.73,P＜0.05).Conclusion:BCAAs may be involved in the development of COPD through inflammatory response,and BCAAs may be potential biomarkers for detecting AECOPD.
关键词
慢性阻塞性肺病/支链氨基酸/细胞因子/炎症机制Key words
Chronic obstructive pulmonary disease/Branched-chain amino acids/Cytokines/Inflammatory pathogenesis引用本文复制引用
基金项目
武汉市卫生健康委科研项目(WX21Q07)
出版年
2024
NETL
NSTL
万方数据