Relationship between expression of CircANKRD36 and level of inflammatory factors and prognosis in patients with septic acute lung injury
Objective:To investigate the relationship between serum circular RNA ankyrin repeat domain 36(CircAN-KRD36)and the levels of inflammatory factors,prognosis in patients with septic acute lung injury.Methods:Totally,112 patients with septic acute lung injury were collected as the lung injury group.According to the 28-day follow-up,they were grouped into a death group of 42 cases and a survival group of 70 cases;another 110 patients with simple sepsis who were treated were regarded as the sepsis group.General data of all patients were collected and compared.The relative expression level of serum CircANKRD36 was detected by real-time quantitative PCR(qRT-PCR).Serum levels of inflammatory factors were detected by enzyme-linked immunosorbent assay(ELISA).Pearson correlation was applied to analyze the relationship between serum CircANKRD36 and inflammatory factor levels in patients in lung injury group.Multivariate logistic regression analysis was applied to analyze the risk factors for poor prognosis in patients with septic acute lung injury.Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of serum CircANKRD36 for poor prognosis in patients with septic acute lung injury.Results:The oxygenation index(OI)in the lung injury group was lower than that in the sepsis group(P＜0.05),and the APACHE Ⅱ score and SOFA score in the lung injury group were higher than those in the sepsis group(all P＜0.05);the OI of the death group was lower than that of the survival group,and the APACHE Ⅱ score,SOFA score and LIPS score in the death group were higher than those in the survival group(all P＜0.05).The levels of serum Cir-cANKRD36,interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),C-reactive protein(CRP)and procalcitonin(PCT)in the lung injury group were higher than those in the sepsis group(all P＜0.05).The levels of serum CircANKRD36,IL-6,TNF-α,CRP and PCT in the death group were higher than those in the survival group(all P＜0.05).Pearson correlation a-nalysis showed that serum CircANKRD36 in the lung injury group was positively correlated with IL-6,TNF-α,CRP,and PCT levels(all P＜0.05).Multivariate Logistic regression analysis showed that low level of OI,high APACHE Ⅱ score,high expressions of CircANKRD36,CRP and PCT were risk factors for poor prognosis in patients with septic acute lung injury(all P＜0.05).The area under curve(AUC)of serum CircANKRD36 for predicting the prognosis of patients with acute lung injury in sepsis group was 0.836,the AUC predicted by the combination of OI,APACHE Ⅱ score,CRP and PCT was 0.963,which was higher than that of each single diagnosis.Conclusion:Serum CircANKRD36 expression is up-regulated in patients with septic acute lung injury,which is closely related to the level of inflammatory factors and prognosis of patients,and can be used as a biomarker for prognosis evaluation of patients with septic acute lung injury.
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