Correlation between serum HIF-1α and lncRNA TUG1 levels and myocardial injury in patients with severe sepsis
Objective:To investigate the correlation between the levels of serum hypoxia inducible factor-1α(HIF-1α)and long non coding RNA taurine up-regulated gene 1(lncRNA TUG1)and myocardial injury in patients with severe sepsis.Methods:A total of 94 patients with sepsis as research objects and patients were grouped into sepsis group(60 cases)and sepsis shock(SS)group(34 cases)according to whether they had shock symptoms at admission.In addition,94 healthy people who had physical examination in our hospital meantime and whose general information matched with sepsis patients were taken as the control group.The expression levels of serum HIF-1α mRNA and lncRNA TUG1 were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction(qRT-PCR).The correlation between serum HIF-1αmRNA,lncRNA TUG1 levels and myocardial injury in SS patients was analyzed by Pearson method.Results:Heart rate,white blood cell(WBC)count and lactic acid in SS group and sepsis group were significantly higher than those in control group,and those in SS group was higher than those in sepsis group(P＜0.05).APACHE Ⅱ score in SS group was higher than that in sepsis group(P＜0.05).The mean arterial pressure,serum levels of HIF-1α mRNA and lncRNA TUG1 in SS group and sepsis group were significantly lower than those in control group,and those in SS group were significantly lower than those in sepsis group(P＜0.05).The percentages of CD3+T,CD4+T,and CD8+T in SS group were obviously lower than those in sepsis group and control group,and those in control group were lower than those in sepsis group(P＜0.05).The levels of lactate dehydrogenase(LD),creatine kinase isoenzyme(CK-MB),cardiac troponin Ⅰ(cTnⅠ),myoglobin(Mb)and heart fatty acid binding protein(H-FABP)in SS group and sepsis group were significantly higher than those in control group and those in SS group were higher than those in sepsis group(P＜0.05).HIF-1α mRNA and IncRNA TUG1 were positively correlated with CD3+T,CD4+T,CD8+T,and negatively correlated with LD,CK-MB,cTnⅠ,Mb,and H-FABP(all P＜0.001).Conclusion:The expression levels of HIF-1α mRNA and lncRNA TUG1 in serum of patients with sepsis are low,both are obviously correlated with myocardial injury in SS patients.
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