Abnormal expression of serum lncRNA XIST and miR-15b increases the risk of acute cerebral infarction
Objective:To investigate the correlation between the expression of long non-coding RNA(lncRNA)X chromosome inactivation specific transcript(XIST)and micrornA-15b(miR-15b)in serum and the risk of acute cerebral infarction.Methods:A total of 146 patients diagnosed with acute cerebral infarction(ACI)by head magnetic resonance ima-ging(MRI)were selected as the observation group,and 80 healthy subjects with no abnormal physical examination during the same period were selected as the control group.At admission,the neurological impairment Scale(NIHSS)of the Nation-al Institutes of Health was used to score the neurological impairment of ACI patients.According to the severity of neurological impairment,ACI patients were divided into a mild group(74 cases),a moderate group(46 cases),and a severe group(26 cases).Serum was collected upon admission,and the expression of serum lncRNA XIST and miR-15b was detected by real-time fluorescent quantitative PCR,and the relationship between their expression and the risk of ACI disease was ana-lyzed.Result:The expression level of serum IncRNA XIST in observation group was significantly lower than that in control group,that in severe group was lower than moderate group and mild group,and that in moderate group was lower than mild group;Serum miR-15b expression level was significantly higher than that of control group,among which severe group was higher than moderate group and mild group,and moderate group was higher than mild group(all P＜0.05).According to Pearson correlation analysis,serum lncRNA XIST expression was negatively correlated with NIHSS score(r=-0.467,P＜0.001).Serum miR-15b expression was positively correlated with NIHSS score(r=0.526,P＜0.001).There were statistically significant differences between patients with high and low expression of serum lncRNA XIST and miR-15b in the scope of cerebral infarction,the type of Oxfordshire Community Stroke Project(OCSP),and dyslipidemia(all P＜0.05).Multivariate logistic regression analysis showed that dyslipidemia(OR=3.380,95％CI:1.338-8.543),serum lncRNA XIST(OR=7.576,95％CI:4.110-13.965),serum miR-15b(OR=8.449,95％CI:5.085-14.036)were independent risk factors for ACI disease occurrence(all P＜0.05).Receiver operating characteristic(ROC)curve results showed that when serum lncRNA XIST expression was 0.72,the area under ROC curve(AUC)for predicting the risk of ACI disease was 0.74(95％CI:2.354-4.212),and the sensitivity and specificity were 77.42％and 70.35％respectively.When serum miR-15b expression was 1.35,the predicted risk of ACI disease was 0.71(95％CI:1.479-3.268),and the sensitivity and specificity were 74.29％and 69.35％respectively.The combined detection predicted the risk of ACI disease with an AUC of 0.78(95％CI:2.527-4.136),sensitivity and specificity of 79.46％and 71.38％respectively.Conclusion:Serum lncRNA XIST and miR-15b expressions are closely related to the degree of neurological impairment in ACI patients.The down-regulation of serum lncRNA XIST expression and up-regulation of miR-15b expression levels are independent risk factors for the risk of ACI disease,and the combined detection of the two has certain reference value for predicting the occur-rence of ACI disease.
Serum long non-coding RNAX chromosome inactivation specific transcriptNon-coding small RNA-15bAcute cerebral infarctionCorrelation
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