Cryopreservation of the shoot of Xanthoceras Sorbifolia Bunge by vitrification was studied. e procedure and corresponding conditions of conservation were as follows: the shoot were subcul-tured by 45 days and detached the size of 2~3mm shoot precultured on MS medium with 0.5M su-crose for 2 days ,then it was dehyrated with 60%PVS2 Solution for 5 minutes at room temperature, then with 100%PVS2 Solution for 30 minutes at 0℃. The materials were plunged into liquid nitro-gen. After 48h,The material was taken off from liquid nitrogen and thawed in 37- 40℃ 2 minute, then it was cultivated on MS+6-BA 0.5mg·L-1+NAA 0.1mg·L-1+GA 0.3 mg·L-1+sucrose 30g·L-1+agar 7g·L-1.
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