Isolation,identification,and application of DSE strain from Artemisia argyi root system and functional bacteria in the rhizosphere
[Objective]To isolate and screen plant growth-promoting strains from the roots and rhizosphere soil of Artemisia argyi.[Methods]To collect root and rhizosphere soil samples of Artemisia argyi from Ordos and Tongliao in Inner Mongolia,isolate and purify symbiotic fungi-dark septate endophytes(DSE)and functional bacteria,identify them using molecular biology techniques,screen DSE strains and functional bacteria with significant growth promoting effects,construct artificial microbial communities,and set up non inoculated bacteria(A),single inoculated DSE(B),single inoculated phosphate-solubilizing bacteria(C),single inoculated nitrogen-fixing bacteria(D),DSE+phosphate-solubilizing bacteria(E),DSE+nitrogen-fixing bacteria(F),phosphate-solubilizing bacteria+nitrogen-fixing bacteria(G),DSE+phosphate-solubilizing bacteria+nitrogen-fixing bacteria(H)eight treatments were used to conduct a rewinding experiment on Artemisia argyi plants to screen for the best bacterial strain.[Results]The colonization rate of DSE fungi in the roots of Artemisia argyi was the highest,and the symbiotic structure was mainly chain,strip,brain and scattered.Four strains of DSE were successfully isolated from the roots of Artemisia argyi,among which Paraphoma chrysanthemicola was the dominant strain in both test sites.A total of 36 functional bacteria were isolated from the rhizosphere soil of Artemisia argyi,including 26 phosphate-solubilizing bacteria and 10 nitrogen-fixing bacteria.The phosphate-solubilizing bacteria were mainly Bacillus,accounting for 30.50%of the total number of isolates,of which Bacillus pumilus accounted for 27.20%.Rhizobium was the main nitrogen-fixing bacteria,accounting for 16.67%of the total number of isolates,of which Rhizobium cellulosilyticum accounted for 33.30%.The growth indexes of Artemisia argyi in B-H treatments were better than those in A treatment.Among them,H treatment had the best growth-promoting effect,and its stem diameter,leaf number,aboveground biomass and underground biomass increased by 24.93%,80.00%,279.17%and 251.69%,respectively.[Conclusion]The compound inoculation of Paraphoma chrysanthemicola,Bacillus pumilus and Rhizobium cellulosilyticum had the best growth-promoting effect,and DSE+phosphate-solubilizing bacteria+nitrogen-fixing bacteria treatment was the best growth-promoting microbial combination.
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