利用HS-SPME-GC-MS法对长叶二裂委陵菜精油成分分析

Compositional analysis of the essential oil of Potentilla bifurca L.var.major Ledeb.by HS-SPME-GC-MS method

夏永怡 ¹杨艳慧 ²徐志伟 ³王瑞刚¹

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作者信息

1. 内蒙古农业大学旱寒区植物逆境适应与遗传修饰改良自治区重点实验室内蒙古呼和浩特 010018
2. 赤峰市翁牛特旗林业和草原局内蒙古乌丹 024000
3. 呼和浩特市赛罕区农牧水利局内蒙古呼和浩特 010000
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摘要

[目的]分析长叶二裂委陵菜精油成分.[方法]利用水蒸气蒸馏法和索氏有机萃取法制备长叶二裂委陵菜精油,采用顶空(HS)-固相微萃取(SPME)-气质联用(GC-MS)法对该精油成分进行分析.[结果]两种制备方法得到的精油中共分离鉴定出 113种挥发性物质成分,主要包含醇类、酯类、醛类、烯烃类、酮类、烷烃类和有机酸化合物,其中,酯类化合物相对含量最高为 50.10%;其次为醇类和烷烃类化合物分别为 39.24%、8.16%.[结论]长叶二裂委陵菜精油挥发性物质的主要组成为酯类、醇类、烷烃类.

Abstract

[Objective]To analyse the components of the essential oil of Potentilla bifurca L.var.major Ledeb..[Methods]The essential oil of Potentilla bifurca L.var.major Ledeb.was prepared by steam distillation and Soxhlet organic extraction,headspace(HS)-solid phase microextraction(SPME)-gas chromatography-mass spectrometry(GC-MS)was used to analyse the components of the essential oils.[Results]A total 113 volatile substance compounds were identified and separated in the essential oils prepared by two methods,mainly including alcohols,esters,aldehydes,olefins,ketones,alkanes and organic acids compounds.Among them,the relative content of ester compounds was the highest at 50.10%;next were alcohols and alkanes,accounting for 39.24%and 8.16%,respectively.[Conclusion]The main volatile substances in the essential oil of Potentilla bifurca L.var.major Ledeb.are esters,alcohols and alkanes.

关键词

HS-SPME-GC-MS法/长叶二裂委陵菜/精油/挥发性物质/成分分析

Key words

HS-SPME-GC-MS method/Potentilla bifurca L.var.major Ledeb/Essential oil/Volatile substances/Component identification

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基金项目

中央引导地方科技发展资金项目(2022ZY0008)

内蒙古自治区高校科技创新团队基本科研业务费项目(NMGIRT2222)

内蒙古自治区高校科技创新团队基本科研业务费项目(BR22-13-05)

呼和浩特市重大科技专项(2022-社-重-1-3)

出版年

2024

北方农业学报

内蒙古自治区农牧业科学院内蒙古自治区农学会

北方农业学报

CSTPCD

影响因子：0.316

ISSN：2096-1197

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