首页|利用分子标记检测59份番茄种质对3种病毒的抗性研究

利用分子标记检测59份番茄种质对3种病毒的抗性研究

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[目的]筛选抗番茄花叶病毒(Tomato masaic virus,ToMV)、番茄斑萎病毒(Tomato spotted wilt virus,TSWV)和番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)的番茄种质,为内蒙古自治区番茄抗病毒育种奠定基础。[方法]以六叶期番茄叶片为试验材料,分析 59份番茄种质抗ToMV、TSWV和TYLCV特性,并进行DNA提取和分子标记检测。[结果]筛选出抗TYLCV的种质 9 份,其中,樱桃番茄 1 种、草莓番茄 1 种、鲜食番茄 7 种;抗TSWV的种质 5 份,其中,樱桃番茄3种、草莓番茄 2种;抗ToMV的种质 14 份,其中,草莓番茄 3种、鲜食番茄 11 种。[结论]59 份番茄种质中,具有病毒抗性的鲜食番茄占 30。51%、草莓番茄占 10。17%、樱桃番茄占 6。78%;具有抗ToMV种质 14 份、抗TSWV种质5 份、抗TYLCV种质 9 份,因此含抗病基因的种质占 47。46%。
Detection of resistance to three viruses in 59 tomato germplasms by molecular markers
[Objective]To screen the tomato germplasm resistance to Tomato masaic virus(ToMV),Tomato spotted wilt virus(TSWV)and Tomato yellow leaf curl virus(TYLCV),and lay a foundation for tomato antiviral breeding in Inner Mongolia Autonomous Region.[Methods]Using six-leaf stage tomato leaves as experimental materials,59 tomato germplasms were analyzed for their resistance to ToMV,TSWV,and TYLCV,and DNA extraction and molecular marker detection were performed.[Results]Nine varieties demonstrated resistance to TYLCV,including 1 cherry tomato,1 strawberry tomato,and 7 fresh tomatoes.Five varieties demonstrated resistance to TSWV,including 3 cherry tomatoes and 2 strawberry tomatoes.Fourteen varieties demonstrated resistance to ToMV,including 3 strawberry tomatoes and 11 fresh tomatoes.[Conclusion]In the 59 tomato germplasms,fresh tomatoes resistant to viruses accounted for 30.51%,strawberry tomatoes accounted for 10.17%,and cherry tomatoes accounted for 6.78%;There were 14 ToMV-resistant germplasms,5 TSWV-resistant germplasms,and 9 TYLCV-resistant germplasms,so 47.46%of the germplasms contained disease resistance genes.

Molecular maker detectionResistance genesTomato mosaic virusTomato spotted wilt virusTomato yellow leaf curl virus

孟彻、高婧、杨永青、付崇毅、廉勇、李正男、刘燕、张磊

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内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特 010018

内蒙古自治区农牧业科学院蔬菜花卉研究所,内蒙古呼和浩特 010031

内蒙古自治区农牧业科学院植物保护研究所,内蒙古呼和浩特 010031

分子标记检测 抗性基因 番茄花叶病毒 番茄斑萎病毒 番茄黄化曲叶病毒

2024

北方农业学报
内蒙古自治区农牧业科学院 内蒙古自治区农学会

北方农业学报

CSTPCD
影响因子:0.316
ISSN:2096-1197
年,卷(期):2024.52(5)