Detection of resistance to three viruses in 59 tomato germplasms by molecular markers
[Objective]To screen the tomato germplasm resistance to Tomato masaic virus(ToMV),Tomato spotted wilt virus(TSWV)and Tomato yellow leaf curl virus(TYLCV),and lay a foundation for tomato antiviral breeding in Inner Mongolia Autonomous Region.[Methods]Using six-leaf stage tomato leaves as experimental materials,59 tomato germplasms were analyzed for their resistance to ToMV,TSWV,and TYLCV,and DNA extraction and molecular marker detection were performed.[Results]Nine varieties demonstrated resistance to TYLCV,including 1 cherry tomato,1 strawberry tomato,and 7 fresh tomatoes.Five varieties demonstrated resistance to TSWV,including 3 cherry tomatoes and 2 strawberry tomatoes.Fourteen varieties demonstrated resistance to ToMV,including 3 strawberry tomatoes and 11 fresh tomatoes.[Conclusion]In the 59 tomato germplasms,fresh tomatoes resistant to viruses accounted for 30.51%,strawberry tomatoes accounted for 10.17%,and cherry tomatoes accounted for 6.78%;There were 14 ToMV-resistant germplasms,5 TSWV-resistant germplasms,and 9 TYLCV-resistant germplasms,so 47.46%of the germplasms contained disease resistance genes.