艾蒿浸提液化感物质的纯化与鉴定

Purification and identification of allelopathic substances from Artemisia annua extract

徐松鹤 ¹田慧英 ¹彭莉原²

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作者信息

1. 集宁师范学院化学与化工学院,内蒙古乌兰察布 012000
2. 集宁师范学院生命科学与技术学院,内蒙古乌兰察布 012000
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摘要

[目的]对艾蒿浸提液化感物质进行纯化与鉴定.[方法]采用纯净水作为溶剂提取艾蒿根、茎、叶中的化学成分,并用GC-MS定性、定量测定其中的酸性、中性和碱性成分.[结果]艾蒿浸提液不同部位检测出的物质不同,其中,酸性成分 38 种,根部所含种类最多(20 种);中性成分 21 种,茎部所含种类最多(10 种);碱性成分 21 种,叶部所含种类最多(9种).根部和茎部含有较多的有机酸类物质与烯类物质,如 5-(1-甲基亚乙基)-1,3-环戊二烯、2,3-二氧代二乙酰基丁腈和顺-双环[4.2.0]辛-3,7-二烯.[结论]艾蒿根和茎可能是其化感物质产生的主要部位,产物多为有机酸类物质与烯类物质.

Abstract

[Objective]To purify and identify the allelopathic substances from Artemisia annua extract.[Methods]The experiment used purified water as a solvent to extract chemical components from the roots,stems,and leaves of Artemisia annua,and quantitatively determined the acidic,neutral,and alkaline components using GC-MS.[Results]Different substances were detected in different parts of Artemisia annua extract,among which 38 acidic components were detected,roots contained the most types(20 types);There were 21 neutral components,stems contained the most types(10 types);There were 21 alkaline components,leaves contained the most types(9 types).The roots and stems contain a significant amount of organic acids and alkenes,such as 5-(1-methylethylene)-1,3-cyclopentadiene,2,3-dioxodiacetylbutyronitrile,and cisbicyclo[4.2.0]octan-3,7-diene.[Conclusion]The roots and stems of Artemisia annua may be the main sites for the production of allelopathic substances,with products mostly consisting of organic acids and alkenes.

关键词

艾蒿/化感物质/纯化与鉴定

Key words

Artemisia annua/Allelopathic substance/Purification and identification

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出版年

2024

北方农业学报

内蒙古自治区农牧业科学院内蒙古自治区农学会

北方农业学报

CSTPCD

影响因子：0.316

ISSN：2096-1197

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