Purification and identification of allelopathic substances from Artemisia annua extract
[Objective]To purify and identify the allelopathic substances from Artemisia annua extract.[Methods]The experiment used purified water as a solvent to extract chemical components from the roots,stems,and leaves of Artemisia annua,and quantitatively determined the acidic,neutral,and alkaline components using GC-MS.[Results]Different substances were detected in different parts of Artemisia annua extract,among which 38 acidic components were detected,roots contained the most types(20 types);There were 21 neutral components,stems contained the most types(10 types);There were 21 alkaline components,leaves contained the most types(9 types).The roots and stems contain a significant amount of organic acids and alkenes,such as 5-(1-methylethylene)-1,3-cyclopentadiene,2,3-dioxodiacetylbutyronitrile,and cisbicyclo[4.2.0]octan-3,7-diene.[Conclusion]The roots and stems of Artemisia annua may be the main sites for the production of allelopathic substances,with products mostly consisting of organic acids and alkenes.
Artemisia annuaAllelopathic substancePurification and identification
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