Effect of continuous cropping on rhizosphere soil microbial communities of spinach
[Objective]To explore the changes in rhizosphere soil physicochemical properties and mirobial community under continuous cropping of spinach.[Methods]The physicochemical properties of the rhizosphere soil of spinach planted for 1 year(1Y)and continuously planted for 2 years(2Y)were measured,and the microbial community in the rhizosphere soil was analyzed using PacBio full-length diversity sequencing.The changes in bacterial and fungal microbial communities in the 2Y and 1Y soils were compared,and the relationship between soil physicochemical properties and microbial communities was studied using redundancy analysis.[Results]Highly significant changes in soil physicochemical properties after continuous cropping(P<0.05),and the pH value and the contents of total nitrogen,total phosphorus,available phosphorus,ammonium nitrogen,organic matter,and microbial biomass carbon were significantly higher in the 2Y soil than in the 1Y soil(P<0.05),the electrical conductivity and the contents of total potassium,available potassium,and nitrate nitrogen were significantly lower than those in the 1Y soil(P<0.05).The redundancy analysis showed that the total potassium was the main factor influencing the rhizosphere soil microbial communities under spinach continuous cropping.The microbial community of the 2Y soil Chao1 index,Richness index,Shannon index,and ACE index were higher than those of 1Y soil.Among which pathogen related to carbon and nitrogen cycle,cellulose degradation,pesticides and plastic degradation and Fusarium associated pathogen content increased significantly in 2Y soil(P<0.05),while the content of Lysobacter biocontrol bacteria with antagonistic activity was significantly decreased(P<0.05).[Conclusion]The rhizosphere soil of spinach under 2Y continuous planting showed significant changes in physicochemical properties,with an increase of pathogen content and a decrease of biocontrol bacteria content.
SpinachContinuous croppingRhizosphere soilPhysicochemical propertyMicrobial community
万方数据
维普
