Screening and identification of cellulose degrading bacteria from Chuzhou Chrysanthemum morifolium(chuju)stem
[Objective]To obtain a strain that can degrade the stem of Chuzhou Chrysanthemum morifolium(chuju)and develop an applied bactericide for stem maturation.[Methods]Samples were taken from the Chuzhou Chrysanthemum morifolium(chuju)planting field in Chuzhou City,Anhui Province,and the primary screening was conducted by Congo red staining and filter paper disintegration test,the cellulase activity and filter paper enzyme activity were rescreened to obtain the strains in situ,the physiological,biochemical and 16S rDNA identification of the strains were performed,the degradation rate of Chuzhou Chrysanthemum morifolium(chuju)stem was determined by filter paper disintegration test and solid state fermentation.[Results]A total of 20 strains capable of degrading cellulose were obtained after screening.Strain N3 was Gram-positive Bacillus with 99.54%homology to Bacillus pumilus.The strain had disintegration effect on filter paper,and the enzyme activity of filter paper was 33.00 U/mL.After 7 days of fermentation culture,the stem degradation rate of Chuzhou Chrysanthemum morifolium(chuju)stem was 41.50%,which was 6.63 times higher than that of the control.[Conclusion]The identified strain N3 was Bacillus pumilus,and has the potential to be developed as a microbial agent for Chuzhou Chrysanthemum morifolium(chuju)stem compost.
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