Study of Fungal Community on the Surface of Ancient Corpses in the Ming Dynasty Based on High-throughput Sequencing Techniques
Based on Illumina MiSeq sequencing technology,this study explored the fungal community structure and diversity in mildew spots on the surface of an ancient corpse of the Ming Dynasty which is preserved in Changzhou Museum.A total of 2 phyla,8 classes,9 orders,10 families,10 genera,and 49 amplicon sequence variants(ASVs)were detected.Aspergillus is the predominant group with a relative abundance of 89.79%-99.88%.ASV1,which belongs to Aspergillus,is the dominant species in the mildew spots with a relative abundance of 55.78%-90.98%.It is preliminarily identified as Aspergillus penicillioides that exceeds the drought tolerance limit,exhibiting absolute dominance in the mildew spots on the surface of the ancient corpse,which may be the main microorganism causing mildew.Identification of microorganisms causing mildew and rot which are produced during the preservation of ancient corpses is helpful to improve the pertinence of mildew prevention and provide reference and a basis for their long-term preservation.
ancient corpsemildewfungusAspergillushigh-throughput sequencing
NETL
NSTL
万方数据
