STUDY ON THE EFFECT OF LOW INTENSITY PULSED ULTRASOUND ON THE NEW STATE OF CONDYLAR CHONDROCYTES
Objective To observe the effect of low-intensity pulsed ultrasound on the expression of phosphorylation of MAPK signaling pathway-related factors in the inflammatory model of rat condylar chondrocytes induced by interleukin-1β.Methods Condylar chondrocytes were isolated by secondary enzymatic digestion and identified.The isolated condylar chondro-cytes were randomly divided into 3 groups,and treated with three different concentrations of IL-1 β,and immunocytochemistry staining of type Ⅱ collagen and MMP-13 was performed to screen out the optimal inflammatory concentration.The extracted rat condylar chondrocytes were randomly divided into 3 groups:NC group,OA group and LIPUS group.After treatment,type Ⅱcol-lagen,p38,p-p38,ERK1/2 and p-ERK1/2 were stained by immunocytochemistry to observe the protein expression.Resu1ts(1)Condylar chondrocytes were successfully isolated.(2)IL-1β:The expression of type Ⅱ collagen in 10 ng/mL group decreased most significantly(P＜0.05),and the expression of MMP-13 increased most significantly(P＜0.05).(3)The expression of typeⅡ collagen in OA group and LIPUS group decreased(P＜0.05),and the decrease in OA group was more obvious(P＜0.05).There was no significant difference in the expression of p38 and ER K1/2 among the three groups(P＞0.05).The expression of p-p38 and p-ERK1/2 in the OA group and the LIPUS group increased(P＜0.05),and the increase in the OA group was more significant(P＜0.05).Conclusions(1)Rat condylar chondrocytes were successfully extracted by secondary enzyme digestion.(2)10 ng/mL was the optimal concentration of IL-1β.(3)LIPUS treatment can reduce the degradation of type Ⅱ collagen in in-flammatory condylar chondrocytes and inhibit the phosphorylation of M APK pathway-related factors p38 and ERK1/2.
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维普
