STUDY ON THE FUNCTION AND MECHANISM OF PLAU IN REGULATING THE METASTASIS OF HUMAN HNSCC
Objective This topic is to study the expression of PLAU in head and neck squamous cell carcinoma and its role in metastasis,and to study its related molecular mechanism.Methods Cultivate human pharyngeal squamous cell carcino-ma cell line FaDu and human laryngeal cancer cell line TU686 in the laboratory.After targeted knockdown/overexpression of the PLAU gene using siRNA,the transfection efficiency was validated using qRT-PCR and Western Blot experiments;Tran-swell and cell scratch assay were used to detect changes in the invasion and migration ability of tumor cells;Detect the expres-sion of N-cadherin,zinc finger transcription protein,and vimentin in HNSCC cells,and analyze their correlation with PLAU in distant tumor metastasis.Results PLAU was highly expressed in HNSCC,and the difference between the groups was statisti-cally significant(P＜0.05),and PLAU in advanced or recurrent metastatic HNSCC was significantly overexpression in early or Carcinoma in situ.The overexpression of PLAU has the ability to promote the metastasis of laryngeal cancer cells.PLAU may regulate the metastasis of head and neck tumors induced by N-cadherin,zinc finger transcription protein,Vimentin,etc.The difference between groups is statistically significant(P＜0.05).Conclusions PLAU may promote the migration and invasion of HNSCC induced cell mesenchymal transition.
Head and neck squamous cell carcinoma(HNSCC)Urokinase type plasminogen activator(PLAU)Me-tastasis
万方数据
维普
