TRIPTOLIDE REGULATES APOPTOSIS OF LUNG CANCER A549 CELLS VIA SIRT2/p53 PATHWAY
Objective To cultivate lung adenocarcinoma A549 cells in vitro,intervene with different concentrations of TP,and investigate apoptosis on lung cancer cells through the SIRT2 pathway,providing new strategies for clinical treatment of lung adenocarcinoma patients.Methods qRT-PCR was used to detect the expression levels of SIRT2-related genes in A549 cells treated with different concentrations of TP(0 nmol/L,60 nmol/L,80 nmol/L).The impact of different concentrations of TP on SIRT2-related proteins in A549 cells was assessed using Western Blot.Results Gene expression level results showed that compared to the control group(0 nmol/L TP),the expression of SIRT2,MDM2 and P300 was downregulated at 60 nmol/L and 80 nmol/L TP.Meanwhile,the expression of the p53 gene was upregulated.Western Blot protein level results demonstrated that at 60 nmol/L and 80 nmol/L TP compared to the control group(0 nmol/L TP),the expression of SIRT2,P300,and MDM2 proteins in A549 cells decreased,while p53 protein expression increased.The Bcl-2 protein expression in the p53 signaling pathway was inhibited,and Bax protein expression was elevated.Conclusions It is proved that TP can inhibits the proliferation and in-duces apoptosis of lung adenocarcinoma cells by regulating the expression of SIRT2/p53 pathway-related proteins.
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