雷公藤甲素调控SIRT2/p53信号通路诱导肺癌A549细胞凋亡
TRIPTOLIDE REGULATES APOPTOSIS OF LUNG CANCER A549 CELLS VIA SIRT2/p53 PATHWAY
宝鲁日 1刘秀兰 1王宇 1徐鸣 1袁宏伟 1李时荣 1王静媛1
作者信息
- 1. 内蒙古医科大学基础医学院,内蒙古 呼和浩特 010059
- 折叠
摘要
目的 体外培养肺腺癌(lung adenocarcinoma,LUAD)细胞A549,采用不同浓度雷公藤甲素(triptolide,TP)干预,研究其通过SIRT2通路诱导肺癌细胞凋亡的作用机制,为临床治疗肺腺癌患者提供新的策略和思路.方法 采用qRT-PCR检测不同浓度(0 nmol/L、60 nmol/L、80 nmol/L)TP对A549细胞中SIRT2相关基因表达水平;不同浓度TP对A549细胞中SIRT2相关蛋白的影响,采用Western Blot法检测.结果 基因表达水平检测结果显示,TP(60 nmol/L、80 nmol/L)与对照组TP(0 nmol/L)相比,SIRT2、MDM2、P300表达下调.同时,p53基因表达上调.Western Blot蛋白水平检测结果,TP(60 nmol/L、80 nmol/L)与对照组TP(0 nmol/L)相比,SIRT2、P300、MDM2蛋白在A549细胞中表达下降,p53蛋白表达上调,p53信号通路的Bcl-2蛋白表达抑制,增加Bax蛋白表达.结论 TP能够通过调控SIRT2/p53通路相关蛋白表达抑制肺腺癌细胞增殖并诱导其凋亡.
Abstract
Objective To cultivate lung adenocarcinoma A549 cells in vitro,intervene with different concentrations of TP,and investigate apoptosis on lung cancer cells through the SIRT2 pathway,providing new strategies for clinical treatment of lung adenocarcinoma patients.Methods qRT-PCR was used to detect the expression levels of SIRT2-related genes in A549 cells treated with different concentrations of TP(0 nmol/L,60 nmol/L,80 nmol/L).The impact of different concentrations of TP on SIRT2-related proteins in A549 cells was assessed using Western Blot.Results Gene expression level results showed that compared to the control group(0 nmol/L TP),the expression of SIRT2,MDM2 and P300 was downregulated at 60 nmol/L and 80 nmol/L TP.Meanwhile,the expression of the p53 gene was upregulated.Western Blot protein level results demonstrated that at 60 nmol/L and 80 nmol/L TP compared to the control group(0 nmol/L TP),the expression of SIRT2,P300,and MDM2 proteins in A549 cells decreased,while p53 protein expression increased.The Bcl-2 protein expression in the p53 signaling pathway was inhibited,and Bax protein expression was elevated.Conclusions It is proved that TP can inhibits the proliferation and in-duces apoptosis of lung adenocarcinoma cells by regulating the expression of SIRT2/p53 pathway-related proteins.
关键词
雷公藤甲素/肺腺癌/A549/SIRT2/p53Key words
Triptolide/lung adenocarcinoma/A549/SIRT2/p53引用本文复制引用
基金项目
内蒙古自治区卫生健康科技计划项目(202201199)
出版年
2024
NETL
NSTL
万方数据