MIR-497 INDUCES HEPATOCELLULAR CARCINOMA CELL LM3 APOPTOSIS THROUGH DOWNREGULATING XIAP
Objective MiR-497 plays a significant role in cell behavior.Bioinformatics analysis showed that miR-497 can bind with the3-UTR of X-linked inhibitor of apoptosis protein(XIAP).This study investigated the role of miR-497 and XIAP in affecting hepatocellular carcinoma(HCC)cell proliferation and apoptosis.Methods There were 75 HCC patients be-tween December 2017 and March 2023 in our hospital enrolled in this study.Tumor tissue and para-carcinoma tissue speci-mens were collected to test miR-497 and XIAP expression.MiR-497 and XIAP levels in normal liver L02 cells and HCC LM3 cells were compared.Ki-67 protein expression and cell apoptosis were detected by flow cytometry.The target relationship be-tween miR-497 and XIAP was verified dual luciferase reporter assay.LM3 cells were divided into mimic NC,miR-497 mimic,si-NC,si-XIAP,and miR-497 mimic+si-XIAP group.Malignant cell growth ability was evaluated by colony formation.Re-sults MiR-497 significantly downregulated,while XIAP expression obviously enhanced in HCC tissue compared with para-carcinoma tissue.MiR-497 reduced and XIAP elevated in LM3 cells compared with L02 cells.LM3 cells presented enhanced cell proliferation and reduced apoptosis.MiR-497 targeted regulated XIAP gene in LM3 cells.MiR-497 upregulation and/or XIAP reduction markedly weakened LM3 cell proliferation and malignant growth,elevated Caspase-3 and Caspase-7 enzyme activity,and promoted cell apoptosis.Conclusions MiR-497 abnormally downregulated,while XIAP enhanced in HCC tis-sue compared with para-carcinoma tissue.MiR-497 may antagonize the inhibitory effect of XIAP on Caspase enzyme activity through targeted suppressing XIAP expression,thus to promote HCC cell apoptosis.
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