摘要
目的 澳洲茄碱对肺癌A549细胞生物活性、瘤体抑制及MAPK/ERK1/2信号的影响.方法 将人肺癌细胞株A549分为空白组、低剂量澳洲茄碱组、中剂量澳洲茄碱组、高剂量澳洲茄碱组及顺铂组,分别加入浓度为0、15、20、25 μmol/L的澳洲茄碱及2 μg/mL顺铂培养.MTT检测活性;流式细胞仪检测凋亡率;Transwell法检测侵袭;划痕试验检测划痕愈合率.25只裸鼠分为空白组、低剂量澳洲茄碱组、中剂量澳洲茄碱组、高剂量澳洲茄碱组及顺铂组,皮下注射0.3 mL的0、15、20、25 µmol/L的澳洲茄碱及2 µg/mL顺铂共培养A549细胞,观察瘤体质量及抑瘤率.结果 澳洲茄碱组降低A549细胞活性,凋亡率升高,侵袭、划痕愈合率、MAPK、p-MAPK、ERK1/2及p-ERK1/2蛋白水平降低(P＜0.05).结论 澳洲茄碱可抑制肺癌细胞增殖、侵袭及转移,并加快凋亡,作用呈现剂量依赖性且高剂量效果最佳,研究机制可能与抑制MAPK/ERK1/2信号表达相关.
Abstract
Objective To investigate the effects of solanine on the biological activity,tumor inhibition and MAPK/ERK1/2 signal of A549 cells of lung cancer.Methods Human lung cancer cell line A549 was divided into blank group,low dose sola-nine group,medium dose solanine group,high dose solanine group and cisplatin group,and cultured with solanine concentra-tion of 0,15,20,25 μmol/L and 2 μg/mL cisplatin,respectively.Activity was measured by MTT;The apoptosis rate was detected by flow cytometry.Invasion was detected by Transwell method.Scratch test was performed to detect the scratch healing rate.25 nude mice were divided into blank group,low-dose solanine group,medium-dose solanine group,high-dose solanine group and cisplatin group.A549 cells were co-cultured with 0.3 mL of solanine 0,15,20,25 μmol/L and 2 μg/mL.Results The activ-ity of A549 cells was decreased,the apoptosis rate was increased,the invasion,scratch healing rate,MAPK,P-MAPK,ERK1/2 and P-ERK1/2 protein levels were decreased(P＜0.05)in solasonine group.Conclusions Solasonine can inhibit the prolifera-tion,invasion and metastasis of lung cancer cells and accelerate apoptosis in a dose-dependent manner,with the best effect at high dose.The mechanism may be related to the inhibition of MAPK/ERK1/2 signal expression.
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