Objective Through the bioinformatics analysis of human skin epidermal cell gene microarray after ultraviolet irradiation,the key genes of photo-damaged dermatosis were obtained and its pathogenesis was explored.Methods Two gene chip data sets of human skin epidermal cell line HaCat exposed to ultraviolet rays were obtained from GEO database.The first gene chip data set was screened for significant differentially ex-pressed genes(DEGs)by R software for GO analysis and KEGG pathway analysis.The other gene chip data set was also screened for DEGs by the same method.A GO and KEGG pathway analysis was conducted at the junc-tion of the two DEGs,with a protein-protein interaction network(PPI)then constructed to acquire essential genes.Results There were 486 DEGs obtained by GSE198792 screening(246 up-regulated and 240 down-regulated)and 90 DEGs obtained by GSE138800 screening(33 up-regulated and 57 down-regulated).20 genes were obtained by intersection screening.GO analysis showed that these differentially expressed genes were mainly related to protein transport,regulation of epidermal growth factor receptor signaling pathway,negative regulation of type I interferon signaling pathway and defense against viruses,and KEGG pathway was mainly related to pentose phosphate pathway.Seven key genes were identified:ISG15,IFI6,TKT,LAP3,SAMHD1,BAG3 and ALDOC.Conclusion Through bioinformatics analysis,the key genes of UV action on human skin epidermal cells were screened,and it was predicted that they may play an important role in skin in-jury caused by UV,which provides a new idea for the prevention and treatment of skin diseases caused by UV.
维普
万方数据