Objective Using CBA method,to detect the serum levels of Th1,Th2 cytokines in patients with T2DM at different pathological stages.Methods A total of 60 T2DM patients diagnosed by the division of nephrology and endocrinology of Heze hospital of traditional Chinese medicine were selected in this study.Study participants were recruited from December 2022 to June 2023.The groups were divided according to continuity and(or)albuminuria in patients with glomerular filtration rate.Diabetes group(DM group):the urine trace al-bumin is negative;Diabetic nephropathy group(DN group):urine microalbumin positive or glomerular filtration rate decreased;21 healthy cases from the same area in same period were selected as the control group(HC group).The serum levels of IFN-γ,TNF,IL-2,IL-4,IL-6,IL-10 were determined using the BD CBA Th1/Th2/Th17 cytokine kit.Results(1)The serum concentrations range of Th1,Th2 cytokines in pa-tients with T2DM at different stages of disease and in healthy controls was obtained by using CBA method.(2)The level of serum IFN-γ in Th1 type cytokines showed a trend of increasing first and then decreasing in HC group,DM group and DN group(P＜0.05);the serum levels of TNF,IL-2,IL-4 and IL-6 had a tendency to increase first and then decrease with the condition;IL-10 level shows a trend of increasing gradually.Con-clusion(1)For the first time,CBA detection method was used to obtain the content ranges of Th1,Th2 cy-tokines in serum of healthy people and patients at different pathological stages of T2DM.(2)The change trend of Th1,Th2 cytokines in the course of T2DM was clarified.(3)Dynamic changes in the overall levels of Th1,Th2 cytokines can be used as important indicators to detect disease progression.(4)Three indicators of IL-2,IL-4 and TNF are closely related to the early stage of T2DM,suggesting that any one of these indicators can be used as the main detection indicator in the early stage of T2DM.However,IL-10 are closely related indicators in the stage of diabetic nephropathy,suggesting that either of these indicators can be used as the main detection in-dicators of the disease or drug efficacy at this stage.
维普
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