Objective To study the effect of dexmedetomidine on postoperative cognitive function in dia-betes rats and its mechanism.Methods Thirty SD rats were randomly divided into control group(CG group),diabetes group(DM group),diabetes+dexmedetomidine group(DM+Dex group),diabetes+dexmedetomi-dine group+methyl niupingting citrate group(DM+Dex+MLA group)and diabetes+dexmedetomidine+LY294002 group(DM+Dex+LY group).The CG group was treated as a normal control group;Diabetes model was established in other groups.The DM+Dex group,DM+Dex+MLA group,and DM+Dex+LY294002 group received intraperitoneal injection of dexmedetomidine before anesthesia;The DM+Dex+MLA group re-ceived intraperitoneal injection of MLA before infusion of dexmedetomidine;The DM+Dex+LY group received intraperitoneal injection of LY294002 before infusion of dexmedetomidine.After anesthesia,each group of rats underwent laparotomy exploration.Conduct a water maze experiment one week after surgery.After euthanizing the rats,the hippocampal tissue was isolated,and the relative expression levels of α 7nAChR,p-PI3K,and p-Akt in the hippocampal tissue were detected by Western blotting.HE staining was used to observe the dam-age to the hippocampal tissue.Compared with the CG group,the Morris water maze experiment in the DM group showed a significant increase in the time required to find the platform(P＜0.05),a significant decrease in the number of platform crossings(P＜0.05),and a significant decrease in the expression of α 7nAChR,p-PI3K,and p-Akt proteins in the hippocampus(P＜0.05).In the DM group,neurons in the hippocampus showed atrophy,necrosis,disordered arrangement,and some neurons had nuclear condensation,deep staining,or even disappearance;Compared with the DM group,the DM+Dex group rats showed a significant decrease in the time required to find the platform(P＜0.05),a significant increase in the number of platform crossings(P＜0.05),a significant increase in the expression of α 7nAChR,p-PI3K,and p-Akt proteins in the hip-pocampus(P＜0.05),and a significant reduction in hippocampal damage compared to the DM group through HE staining;The effect of dexmedetomidine can be partially reversed by inhibitors of α 7nAChR,namely MLA and PI3K inhibitors LY294002.Conclusion Dextrmedetomidine can improve the postoperative cognitive func-tion of diabetes rats through the PI3K Akt signal pathway mediated by α 7 nAChR.
维普
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