The expression characteristics and functional analysis of KMT2C in gastric cancer
Objective:To investigate the expression,function,and clinical significance of histone lysine(k)-specific methyltr-ansferase 2C(KMT2C)in gastric cancer.Methods:Quantitative real-time PCR was used to detect the expression of KMT2C in 150 gastric cancer tissues and corresponding normal tissues.Chi-square test,Kaplan-Meier survival analysis,and Cox regression model were employed to analyze the correlation between KMT2C expression and clinicopathological features and prognosis in gastric cancer patients.CCK-8 experiment,colony formation experiment,wound healing experiment and Transwell migration and invasion experiment were performed to evaluate the impact of KMT2C knockdown on the proliferation,migration,and invasion abilities of gastric cancer cells.Results:The expression of KMT2C mRNA in 98 gastric cancer tissues was higher than that in normal tissues.Correlation analysis showed that KMT2C mRNA expression was significantly correlated with TNM stage(P=0.032)and T stage(P=0.044).Multivariate Cox regression analysis demonstrated that KMT2C mRNA expression level(HR=1.693,95％CI:1.034-2.774,P=0.037)and TNM stage(HR=1.903,95％CI:1.127-3.212,P=0.016)were independent risk factors affecting prognosis of gastric cancer.Overall survival was significantly lower in patients with high KMT2C expression compared to those with low expression(P＜0.05).Furthermore,knockdown of KMT2C expression inhibited proliferation,migration,and invasion abilities of gastric cancer cells(SGC-7901).Conclusion:KMT2C is highly expressed as an oncogene in gastric cancer and is associated with poor prognosis.KMT2C may be a potential target for the treatment of gastric cancer.
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