MAGEA12 expression in gastrointestinal stromal tumors and its relationship to risk classification
Objective:To investigate the expression level of melanoma-associated antigen gene A12(MAGEA12)in gastroin-testinal stromal tumors(GIST)and its relationship with GIST risk classification.Methods:The tumor tissues and adjacent normal tissues of 5 patients with GIST were collected.Principal components analysis(PCA),gene set enrichment analysis(GSEA)and differential expression gene analysis were used to identify the abnormal signaling pathways and abnormal expressed genes in GIST tissues by transcriptome sequencing.Quantitative real-time PCR(qRT-PCR)was used to verify the expression level of MAGEA12 in 90 pairs of GIST tissues.Chi-square test was used to analyze the correlation between the expression level of MAGEA12 and the clinicopathological characteristics of GIST patients.Results:PCA analysis showed that the gene expression profile of GIST tissue was significantly different from that of adjacent cancer control tissue.GSEA analysis found that a variety of tumor-related signaling pathways in GIST tissues were abnormally highly expressed.Differential expression gene analysis showed that MAGEA12 was the gene with the highest upregulation in GIST tissues(25-fold increase in expres-sion).QRT-PCR verified that MAGEA12 was significantly higher expressed in GIST samples and was positively correlated with GIST risk classification.Conclusion:MAGEA12 is highly expressed in GIST,its expression level is correlated with GIST risk,and may become a new target for GIST therapy.
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