Study on the biocompatibility of spinal cord high biomimetic scaffold 3D printed with chitosan and gelatin composite bioink
Objective:To explore the formulation and biocompatibility and reliability evaluation of composite bioink for 3D printed spinal cord bionic stent.Methods:Chitosan(C)and gelatin(G)were used as composite bioink by 3D printing technology.The physicochemical properties of composite bioinks with different ratios were compared,including porosity,swelling rate,rheological properties,degradation rate and mechanical properties.By optimizing the printing parameters,the spinal cord shape bionic stent with specific spatial configuration was printed.Then,the bionic scaffold extract was co-cultured with SH-SY5Y cells,and the cytotoxicity of the scaffold and its effect on cell proliferation were evaluated by scanning electron microscopy and CCK-8 assay.Results:By comparing the final selection of 4%concentration of C and 6%concentration of G(4C6G)group as the most suitable compound bioink formulation,and the follow-up study was carried out.Under the parameters of printing speed of 1.5 mm/s,pressure of 0.09-0.11 MPa,layer thickness of 0.1 mm,spacing of 1 mm and needle of 0.11 mm,the butterfly shape high-fidelity spinal cord bionic scaffold of 5 mm×5 mm×0.9 mm can be printed.Scanning electron microscopy showed that SH-SY5Y cells adhered to the interior of the scaffold and could grow normally.CCK-8 results showed that the extract had no cytotoxicity.Conclusion:C and G bioink is expected to be the raw material of bionic stent for spinal cord injury repair.
3D printingchitosangelatintissue engineeringspinal cord bionic stent
NETL
NSTL
万方数据
