Preliminary study on circRNAs expression profile of radioresistant cervical squamous cell carcinoma
Objective:To analyze the differential expression of circular RNAs(circRNAs)in the tumor tissues of patients with cervical squamous cell carcinoma(CSCC)that are sensitive to and resistant to radiotherapy.Methods:The Illumina PE150 high-throughput sequencing technology was used to detect the differentially expressed circRNAs in tumor tissues of 3 radioresistant and 3 radiosensitive CSCC patients,and information mining and analysis of the sequence were carried out.The 60 differential expression of circRNAs were verified by quantitative real time-polymerase chain reaction(qRT-PCR).Results:There were 1 316 circRNAs that were twice or more differentially expressed between radiosensitive and radioresistant CSCC tissues.Compared with radiosensitive CSCC tissues,there were 594 differential circRNAs that were significantly up-regulated in radioresistant CSCC tissues,and 722 differential circRNAs were significantly down-regulated.Gene Ontology(GO)analysis showed that in terms of cell biological processes,differential circRNAs are mainly enriched in cellular metabolic processes,organelles,primary metabolic processes,heterocyclic metabolic processes,etc.In terms of molecular functions,differential circRNAs are mainly enriched in protein binding,kinase binding,chromatin binding,heterocyclic compound binding,etc.Enrichment analysis of Kyoto encyclope-dia of genes and genomes(KEGG)signaling pathway revealed that differential circRNAs were enriched in thyroid hormone signaling pathway,Hippo signaling pathway,FoxO signaling pathway and other pathways.qRT-PCR analysis showed that hsa circ 0003373,hsa circ 0004337,hsa circ 0005114,hsa circ 0031431,novel circ 0006491,novel circ 0070688 and novel circ 0070695 were significantly down-regulated in radioresistant CSCC tissues compared with radiosensitive CSCC tissues(all P<0.05).Conclusions:Hsa circ 0003373,hsa circ 0004337,hsa circ 0005114,hsa circ 0031431,novel circ 0006491,novel circ 0070688 and novel circ 0070695 were significantly down-regulated in radioresistant CSCC tissues compared with radiosensitive CSCC tissues,which provides an experimental basis for further study on the role of circRNAs in CSCC radiotherapy resistance.
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