目的:比较不同给氧方案对脂多糖(lipopolysaccharide,LPS)诱导的急性肾损伤(acute kidney injury,AKI)模型小鼠肾组织炎症性损伤及外周血炎症因子的影响.方法:采用ICR雌性小鼠,腹腔注射LPS(5 mg/kg)建立小鼠AKI模型,随机分成:(1)空白组(Control组),注射同等量的生理盐水;(2)模型组(LPS组);(3)纯氧治疗组(LPS+100%O2 组);(4)2.0绝对大气压(atmosphere absolute,ATA)高压氧(hyperbaric oxygen,HBO)治疗组(LPS+2.0 ATA HBO组);(5)3.0 ATA HBO治疗组(LPS+3.0 ATA HBO组),治疗后 24 h,通过流式微球技术及实时荧光定量PCR(real-time PCR,RT-PCR)实验分别检测肾组织和外周血中促炎因子IL-1β、IL-6 和TNF-α,以及抑炎因子IL-10 水平.此外,通过HE染色法观察肾组织炎症性损伤的组织形态变化.结果:IL-1β、IL-6 和TNF-α在LPS诱导 24h后均显著增加;100%O2 和 2.0 ATA HBO治疗均可抑制促炎因子IL-1β、IL-6 和TNF-α的释放,且 2.0 ATA HBO抑制作用更显著(P<0.05 或P<0.01);此外,LPS+2.0 ATA HBO组抑炎因子IL-10 水平显著增高(P<0.01).HE染色结果表明,与LPS组相比,100%O2 和 2.0 ATA HBO治疗后,肾组织炎症性损伤均有所减轻,且LPS+2.0 ATA HBO组改善效果最好(P<0.05 或P<0.01),而LPS+3.0 ATA HBO组肾组织损伤未改善.结论:100%O2 和 2.0 ATA HBO治疗均可明显改善AKI小鼠炎症性损伤,且 2.0 ATA HBO改善作用更好.
Comparison of the protective effects of different oxygen therapy regimens against acute kidney injury in mice
Objective:Comparison of the effects of different oxygen therapy regimens on renal tissue inflammatory injury and peripheral blood inflammatory factors in a mouse model of lipopolysaccharide(LPS)-induced acute kidney injury(AKI).Meth-ods:ICR female mice were injected intraperitoneally with 5 mg/kg of LPS to establish a model of AKI.They were randomly divided into the following groups:(1)Control group(injected with the same amount of physiological saline);(2)Model group(LPS group);(3)Pure oxygen treatment group(injected with LPS and 100%O2);(4)2.0 atmosphere absolute(ATA)hyperbaric oxygen(HBO)treatment group(LPS+2.0 ATA HBO group);(5)3.0 ATA HBO treatment group LPS+3.0 ATA HBO group).The pro-inflammatory cytokines IL-1β,IL-6,and TNF-α and the anti-inflammatory cytokine IL-10 in peripheral blood and kidney tissue were detected by flow cytometric bead array and real-time PCR(RT-PCR)assays.The histomorphological changes of kidney tissue were observed by HE staining.Results:IL-1β,IL-6,and TNF-α levels were significantly increased after 24 hours of LPS induction.100%O2 and 2.0 ATA HBO could inhibit the release of IL-1β,IL-6,and TNF-α,and 2.0 ATA HBO had a more pronounced impact,with a significantly increased IL-10 level in this treatment group.HE staining showed that compared with LPS,100%O2 and 2.0 ATA HBO could alleviate the inflammatory injury of kidney,and 2.0 ATA HBO showed a better effect.Nevertheless,3.0 ATA HBO did not reverse the damage to the kidney tissue.Conclusion:The above results suggested that both 100%O2 and 2.0 ATA HBO treatments significantly improved inflammatory injury in AKI mice.Moreover,2.0 ATA HBO had a superior effect on inhibiting tissue inflammation.
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